IL-6 Monoclonal Antibody (MP5-20F3), PE, eBioscience™, Invitrogen™

Description

• Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6)
• The MP5-20F3 antibody is a neutralizing antibody
• Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells
• Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6
• This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction
• Applications Reported: This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This MP5-20F3 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages
• IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells
• The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections
• The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha
• The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis
• Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2)
• Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.