IL-6 Monoclonal Antibody (MQ2-13A5), FITC, eBioscience™, Invitrogen™

Description

• Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types
• Applications Reported: MQ2-13A5 has been reported for use in intracellular flow cytometric analysis
• Applications Tested: This MQ2-13A5 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells
• The FITC conjugated MQ2-13A5 is offered in 2 formats: - μg size: This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• - test size: has been pre-titrated and tested by intracellular flow cytometric analysis of stimulated normal human perippheral blood cells
• This can be used at test size: 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells
• The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections
• The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha
• The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis
• Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2)
• Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.