7069582
TNF alpha Monoclonal Antibody (TN3-19.12), eBioscience™, Invitrogen™
Armenian Hamster Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
TNF alpha
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols
Tnf
Primary or Secondary
Primary
Content And Storage
4° C
Gene
Tnf
Clone
TN3-19.12
Applications
ELISA, Flow Cytometry, Functional Assay, Neutralization
Conjugate
Unconjugated
Host Species
Armenian Hamster
Target Species
Mouse, Rat
Gene Accession No.
P06804, P16599
Gene ID (Entrez)
21926, 24835
Isotype
IgG
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha
- The TN3-19.12 antibody is a neutralizing antibody
- Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells
- A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule
- TNF alpha is cytolytic and plays an important role in immune regulation
- Dimers and trimers of TNF alpha have been observed
- Applications Reported: The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, ELISPOT, IVCCA, and ELISA
- Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry
- Functional Grade purified TN3-19.12, cat
- 16-7423, is recommended for use in functional assays
- For ELISPOT capture, the alternative clone, 1F3F3D4 (cat No
- 16-7325), is recommended
- Applications Tested: The TN3-19.12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the biotinylated anti-mouse/rat TNF alpha polyclonal antibody (13-7341) for detection and recombit mouse TNF alpha (14-8321) or rat TNF alpha as the standard
- Product 14-8320 is a bioactive protein, but is not recommended for rat TNF alpha ELISA.] A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
- TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
- This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
- TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
- TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
- Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
- Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
- TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
- This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Compare Similar Items
Show Difference
Antigen: TNF alpha
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols: Tnf
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Tnf
Clone: TN3-19.12
Applications: ELISA, Flow Cytometry, Functional Assay, Neutralization
Conjugate: Unconjugated
Host Species: Armenian Hamster
Target Species: Mouse, Rat
Gene Accession No.: P06804, P16599
Gene ID (Entrez): 21926, 24835
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
TNF alpha
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols:
Tnf
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Tnf
Clone:
TN3-19.12
Applications:
ELISA, Flow Cytometry, Functional Assay, Neutralization
Conjugate:
Unconjugated
Host Species:
Armenian Hamster
Target Species:
Mouse, Rat
Gene Accession No.:
P06804, P16599
Gene ID (Entrez):
21926, 24835
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: FOXP3
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
Gene Symbols: Foxp3
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Foxp3
Clone: eBio7979 (221D/D3)
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Mouse
Gene Accession No.: Q99JB6, Q9BZS1
Gene ID (Entrez): 20371, 50943
Isotype: IgG1
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
FOXP3
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
Gene Symbols:
Foxp3
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Foxp3
Clone:
eBio7979 (221D/D3)
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Mouse
Gene Accession No.:
Q99JB6, Q9BZS1
Gene ID (Entrez):
20371, 50943
Isotype:
IgG1
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Granzyme B
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
Gene Symbols: GZMB
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: GZMB
Clone: 16G6
Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rat
Target Species: Human, Mouse
Gene Accession No.: P04187, P10144
Gene ID (Entrez): 14939, 3002
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Granzyme B
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
Gene Symbols:
GZMB
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
GZMB
Clone:
16G6
Applications:
ELISA, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Human, Mouse
Gene Accession No.:
P04187, P10144
Gene ID (Entrez):
14939, 3002
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Cytokeratin 7
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: CK7; CK-7; Cytokeratin 7; cytokeratin-7; D15Wsu77e; K2C7; K7; Kb7; keratin 7; keratin 7, type II; keratin associated protein 7.1; keratin complex 2, basic, gene 7; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 7; Keratin-7; keratin-associated protein 7-1; keratin-associated protein 7-1-like protein; Krt2-7; KRT7; krt7 {ECO:0000250; LOC100755511; LOC100861181; NACHT, LRR and PYD domains-containing protein 3; sarcolectin; SCL; si:ch73-233m11.1; si:ch73-233m11.2; similar to keratin 7; type II keratin Kb7; type II mesothelial keratin K7; type-II keratin Kb7; UniProtKB:P08729}
Gene Symbols: KRT7
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: KRT7
Clone: LP5K
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P08729
Gene ID (Entrez): 3855
Isotype: IgG2b
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Cytokeratin 7
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
CK7; CK-7; Cytokeratin 7; cytokeratin-7; D15Wsu77e; K2C7; K7; Kb7; keratin 7; keratin 7, type II; keratin associated protein 7.1; keratin complex 2, basic, gene 7; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 7; Keratin-7; keratin-associated protein 7-1; keratin-associated protein 7-1-like protein; Krt2-7; KRT7; krt7 {ECO:0000250; LOC100755511; LOC100861181; NACHT, LRR and PYD domains-containing protein 3; sarcolectin; SCL; si:ch73-233m11.1; si:ch73-233m11.2; similar to keratin 7; type II keratin Kb7; type II mesothelial keratin K7; type-II keratin Kb7; UniProtKB:P08729}
Gene Symbols:
KRT7
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
KRT7
Clone:
LP5K
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P08729
Gene ID (Entrez):
3855
Isotype:
IgG2b
Purification Method:
Affinity chromatography
Product Type:
Antibody