TNF alpha Monoclonal Antibody (MP6-XT22), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4^+ T cells
• TNF-alpha has cytolytic activity against a range of tumor cells and is important in immune regulation
• TNF-alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form
• Applications Reported: The MP6-XT22 antibody has been reported for use in ELISA, neutralization, and immunohistochemistry
• Applications Tested: The Functional Grade Purified MP6-XT22 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for neutralization of TNF alpha bioactivity
• The MP6-XT22 antibody at 1.25 μg/mL has been found to neutralize by 50% the biological effects of 1.0 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells
• Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
• This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
• TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
• TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
• Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
• Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
• TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
• This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.