MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s)
• It detects a polymorphic determit present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes
• The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes
• Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis and in vitro blocking of T cell proliferative responses
• Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells
• Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells
• The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.