IL-6 Monoclonal Antibody (MP5-20F3), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6)
• The MP5-20F3 antibody is a neutralizing antibody
• Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells
• Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6
• This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction
• Applications Reported: This MP5-20F3 antibody has been reported for use in cytokine neutralization, ELISA capture antibody, and ELISPOT capture antibody
• Applications Tested: The Functional Grade Purified MP5-20F3 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-6 bioactivity
• The MP5-20F3 antibody at 0.03 μg/mL has been found to inhibit by 50% the biological effects of 0.01 ng/mL mouse IL-6 in an B9 cell proliferation assay
• Detailed information and protocols about can be found in the BestProtocols™ section
• The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-6 (IL-6) in combination with the biotinylated MP5-32C11 (cat
• 13-7062) antibody for detection and recombit mouse IL-6 (cat
• IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells
• The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections
• The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha
• The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis
• Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2)
• Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.