CD369 (Clec7a, Dectin-1) Monoclonal Antibody (15E2), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A
• CD369 is expressed predomitly on monocytes, macrophages, and dendritic cells
• Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS
• In humans, CD369 exists as two isoforms that arise due to alternative splicing
• CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections
• Studies have also shown the involvement of CD369 in dendritic cell activation
• The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells
• It does not crossreact to mouse CD369
• Applications Reported: The 15E2 antibody has been reported to activate dendritic cells
• Applications Tested: This 15E2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0
• CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells
• It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM)
• CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response
• Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.