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7078507

NK1.1 Monoclonal Antibody (PK136), Super Bright™ 600, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

NK1.1

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias

AI462337; CD161; CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; EMBL:AAQ11375.1}; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; killer cell lectin-like receptor subfamily B member 1F; KLRB1; Klrb1b; klrb1b {ECO:0000312; Klrb1c; Klrb1d; Klrb1f; klrb1f {ECO:0000250; Ly55; Ly-55; Ly55b; ly-55b; Ly55c; ly-55c; Ly55d; Ly-55d; Ly59; Ly-59; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; natural killer cell surface protein NKR-P1B allele B6; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele SJL/BALB; natural killer cell surface protein NKR-P1B allele TO; natural killer cell surface protein NKR-P1F; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; Nk1; Nk-1; NK1.1; Nk1.2; Nk-1.2; NKRP1; NK-RP1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKRP140; Nkrp1b; NKR-P1B; Nkrp1-b; NKR-P1B protein; Nkrp1c; NKR-P1C; Nkrp1d; NKR-P1D; Nkrp1f; NKR-P1F; RGD:2975}; UniProtKB:Q8VD98}

Gene Symbols

Klrb1b, Klrb1c

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

PK136

Applications

Flow Cytometry

Conjugate

Super Bright 600

Host Species

Mouse

Target Species

Mouse

Gene Accession No.

P27812, P27814

Gene ID (Entrez)

17059, 80782

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB
  • Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen
  • For detection of NK cells in these strains the monoclonal antibody DX5 (Cat
  • No
  • 14-5971) should be used
  • Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells
  • Applications Reported: This PK136 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This PK136 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
  • We recommend using a 610/20 bandpass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation
  • Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function
  • The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain
  • The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus
  • NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

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Mouse Monoclonal Antibody...

Antigen:
NK1.1
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
AI462337; CD161; CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; EMBL:AAQ11375.1}; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; killer cell lectin-like receptor subfamily B member 1F; KLRB1; Klrb1b; klrb1b {ECO:0000312; Klrb1c; Klrb1d; Klrb1f; klrb1f {ECO:0000250; Ly55; Ly-55; Ly55b; ly-55b; Ly55c; ly-55c; Ly55d; Ly-55d; Ly59; Ly-59; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; natural killer cell surface protein NKR-P1B allele B6; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele SJL/BALB; natural killer cell surface protein NKR-P1B allele TO; natural killer cell surface protein NKR-P1F; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; Nk1; Nk-1; NK1.1; Nk1.2; Nk-1.2; NKRP1; NK-RP1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKRP140; Nkrp1b; NKR-P1B; Nkrp1-b; NKR-P1B protein; Nkrp1c; NKR-P1C; Nkrp1d; NKR-P1D; Nkrp1f; NKR-P1F; RGD:2975}; UniProtKB:Q8VD98}
Gene Symbols:
Klrb1b, Klrb1c
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
PK136
Applications:
Flow Cytometry
Conjugate:
Super Bright 600
Host Species:
Mouse
Target Species:
Mouse
Gene Accession No.:
P27812, P27814
Gene ID (Entrez):
17059, 80782
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody

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Concentration:
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Classification:
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Form:
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Formulation:
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Gene Alias:
AI467657; Green's luxoid; lu; luxoid; Lx; PLZF; Polydactyly-luxate syndrome; promyelocytic leukaemia zinc finger; promyelocytic leukemia zinc finger; Promyelocytic leukemia zinc finger protein; ZBTB16; Zfp145; zinc finger and BTB domain containing 16; zinc finger and BTB domain containing 16 protein; zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); zinc finger protein PLZF; ZNF145
Gene Symbols:
ZBTB16
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
9E12
Applications:
Flow Cytometry
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PE-Cyanine7
Host Species:
Armenian Hamster
Target Species:
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Purification Method:
Affinity chromatography
Product Type:
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Concentration:
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Form:
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Regulatory Status:
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Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Gene Symbols:
__
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
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Control, Flow Cytometry
Conjugate:
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Host Species:
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Target Species:
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Gene Accession No.:
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Gene ID (Entrez):
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Antigen:
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Concentration:
0.2 mg/mL
Classification:
__
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Gene Symbols:
__
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
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Control, Flow Cytometry
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Super Bright 600
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Target Species:
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Gene Accession No.:
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