PLZF Monoclonal Antibody (9E12), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors
• Expression of this transcriptional repressor in immune cells differs between mice and humans
• In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells
• Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells
• In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells
• PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans
• PLZF is expressed by innate lymphoid cells and its progenitor cells
• PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintece of homeostatis
• Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor
• This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7
• This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat
• 00-5523) and protocol
• This can be used at less than or equal to 0
• This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus
• This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase
• Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL)
• Alternate transcriptional splice variants have been characterized.