LAG-3 (CD223) Mouse anti-Human, APC-R700, Clone: T47-530, BD

Description

• The T47-530 specifically recognizes the Lymphocyte Activation Gene 3 (LAG-3) protein which is also known as, Protein FDC, or CD223
• LAG-3 is a ñ70 kDa type I transmembrane glycoprotein that belongs to the Ig superfamily and exhibits homology to CD4
• LAG-3 is expressed on NK cells, regulatory T cells, and activated conventional T cells with higher expression found on CD8+ T cells compared with CD4+ T cells
• LAG-3 is an activation induced cell surface molecule that like CD4, binds MHC class II molecules, but with much higher affinity
• This may enable LAG-3 to act as a negative competitor of CD4 for MHC class II ligand binding
• LAG-3 may associate with the TCR-CD3 complex to downregulate TCR signal transduction and T cell clonal expansion
• In contrast, LAG-3-induced signaling may promote dendritic cell activation
• This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700
• APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness
• This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45nm filter)
• APC-R700 is a tandem fluorochrome that combines APC with R700, a proprietary organic dye
• This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor™ 700
• Due to similar excitation and emission properties, APC-R700 and Alexa Fluor™ 700 cannot be used simultaneously.