MP Biomedicals™ β-Nicotinamide Adenine Dinucleotide, Oxidized Form, MP Biomedicals™

Description

• Description β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes
• It occurs in living cells primarily in the oxidized state
• It serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols
• NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain
• Electron acceptor
• β-NAD is a carrier for the hydride ion, forming b-NADH
• Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase
• Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH
• Unlike b-NAD which has no absorbance at 340nm, b-NADH absorbs at 340nm (EmM = 6.22)
• The increase in absorbance at 340nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.