MP Biomedicals™ β-Nicotinamide Adenine Dinucleotide, Oxidized, MP Biomedicals™

Description

• Many metabolites and enzymes of biological interest are present in tissues at low concentrations
• With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved
• The reduced form, β-NADH, is fluorescent whereas β-NAD is not
• This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M
• Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols NAD usually acts as an electron acceptor, acting as a carrier for hydride ion, forming b-NADH which then serves as a hydrogen donor in the respiratory chain Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH Unlike b-NAD which has no absorbance at 340nm, b-NADH absorbs at 340nm (EmM = 6.22) The increase in absorbance at 340nm with the formation of b-NADH is the basis for measurement of activity of many enzymes