7090323

CD23 Monoclonal Antibody (B3B4), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD23

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

BLAST-2; CD23; CD23 antigen; CD23A; CLEC4J; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon receptor II; FC epsilon RII; Fc fragment of IgE receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc receptor alpha multiple ligand receptor; Fc receptor, IgE, low affinity II, alpha polypeptide; Fcalpha muR; Fcalpha RII; Fce2; fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; low affinity immunoglobulin epsilon Fc receptor; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; low-affinity IgE receptor; Ly-42; lymphocyte IgE receptor

Gene Symbols

Fcer2a

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Fcer2a

Clone

B3B4

Applications

Flow Cytometry

Conjugate

PE-Cyanine7

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P20693

Gene ID (Entrez)

14128

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein
  • CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation
  • B-1 cell lineage (CD5+ B cells) does not express CD23
  • Soluble forms of the antigen have been reported to be biologically active
  • CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation
  • CD21 is thought to bind to CD23
  • Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
  • The CD23 molecule is identical to the low affinity IgE receptor found on B cells
  • Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma
  • CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
  • Functionally, CD23 is involved in B cell growth and differentiation, and IgE production
  • Further, CD23 has a soluble form that is a potent mitogenic factor
  • Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

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Rat Monoclonal Antibody...


Antigen:
CD23

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
BLAST-2; CD23; CD23 antigen; CD23A; CLEC4J; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon receptor II; FC epsilon RII; Fc fragment of IgE receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc receptor alpha multiple ligand receptor; Fc receptor, IgE, low affinity II, alpha polypeptide; Fcalpha muR; Fcalpha RII; Fce2; fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; low affinity immunoglobulin epsilon Fc receptor; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; low-affinity IgE receptor; Ly-42; lymphocyte IgE receptor

Gene Symbols:
Fcer2a

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Fcer2a

Clone:
B3B4

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P20693

Gene ID (Entrez):
14128

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD24

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
CD24; CD24 antigen; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 molecule; CD24A; CD24a antigen; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; heat-stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; nectadrin heat stable antigen; R13-Ag; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; X62 heat stable antigen

Gene Symbols:
Cd24a

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd24a

Clone:
M1/69

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P24807

Gene ID (Entrez):
12484

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD25

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR

Gene Symbols:
IL2RA

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IL2RA

Clone:
PC61.5

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P01590

Gene ID (Entrez):
16184

Isotype:
IgG1 λ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD25

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR

Gene Symbols:
IL2RA

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IL2RA

Clone:
CD25-4E3

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Mouse

Target Species:
Human, Non-human Primate

Gene Accession No.:
P01589

Gene ID (Entrez):
3559

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody