7090367

CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine7, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD69

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

5830438K24Rik; Activation inducer molecule; activation inducer molecule (AIM/CD69); AI452015; AIM; BL-AC/P26; CD69; CD69 antigen; CD69 antigen (p60, early T-cell activation antigen); CD69 molecule; CLEC2C; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; early activation antigen CD69; early lymphocyte activation antigen; early T-cell activation antigen p60; GP32/28; leukocyte surface antigen Leu-23; MLR-3; VEA; Very Early Activation Antigen

Gene Symbols

CD69

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

CD69

Clone

H1.2F3

Applications

Flow Cytometry

Conjugate

PE-Cyanine7

Host Species

Armenian Hamster

Target Species

Mouse

Gene Accession No.

P37217

Gene ID (Entrez)

12515

Isotype

IgG

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA)
  • CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer
  • While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation
  • Applications Reported: The H1.2F3 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions
  • CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets
  • In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction
  • Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation
  • Furthermore, the protein may act to transmit signals in natural killer cells and platelets
  • Alternative splicing results in multiple transcript variants of CD69
  • Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

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Show Difference

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Antigen:
CD69

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
5830438K24Rik; Activation inducer molecule; activation inducer molecule (AIM/CD69); AI452015; AIM; BL-AC/P26; CD69; CD69 antigen; CD69 antigen (p60, early T-cell activation antigen); CD69 molecule; CLEC2C; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; early activation antigen CD69; early lymphocyte activation antigen; early T-cell activation antigen p60; GP32/28; leukocyte surface antigen Leu-23; MLR-3; VEA; Very Early Activation Antigen

Gene Symbols:
CD69

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD69

Clone:
H1.2F3

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Armenian Hamster

Target Species:
Mouse

Gene Accession No.:
P37217

Gene ID (Entrez):
12515

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD71 (Transferrin Receptor)

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
2610028K12Rik; AI195355; AI426448; AU015758; CD antigen CD71; CD71; chicken transferrin receptor; E430033M20Rik; I79_000642; IMD46; mammary tumor virus receptor 1; Mtvr1; Mtvr-1; p90; sTfR; T9; TfR; tfR1; TFR2; TFRC; TR; transferrin receptor; transferrin receptor (p90, CD71); transferrin receptor 2; transferrin receptor protein 1; Transferrin receptor protein 1, serum form; Trfr

Gene Symbols:
TFRC

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
TFRC

Clone:
OKT9 (OKT-9)

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P02786

Gene ID (Entrez):
7037

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD73

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
2210401F01Rik; 5 nucleotidase, ecto; 5' nucleotidase, ecto; 5'-NT; 5'-nucleotidase; 5'-nucleotidase ecto; 5'-nucleotidase, ecto (CD73); AI447961; CALJA; CD73; E5NT; ecto-5'-nucleotidase; eN; eNT; NT; Nt5; Nt5e; NTE; Purine 5-Prime-Nucleotidase

Gene Symbols:
NT5E

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
NT5E

Clone:
eBioTY/11.8 (TY/11.8)

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q61503

Gene ID (Entrez):
23959

Isotype:
IgG1

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD80 (B7-1)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Gene Symbols:
CD80

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD80

Clone:
16-10A1

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Armenian Hamster

Target Species:
Canine, Mouse, Porcine

Gene Accession No.:
Q00609

Gene ID (Entrez):
12519, 397161, 403765

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody