CD4 Monoclonal Antibody (RM4-5), eFluor™ 615, eBioscience™, Invitrogen™

Description

• Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells
• CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells
• In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail
• Binding of RM4-5 is blocked by GK1.5
• Applications Reported: This RM4-5 antibody has been reported for use in immunocytochemical and immunohistochemical staining of frozen tissue sections (IHC-F)
• Applications Tested: This Rm4-5 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 1 μg/mL
• It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest
• This product has not been validated for flow cytometric analysis
• Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75)
• A standard Alexa Fluor™ 594 filter is acceptable
• Excitation: 595 nm; Emission: 615 nm
• Filtration: 0.2 μm post-manufacturing filtered
• The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
• CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
• At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
• CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
• It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].