7090849

TIM-4 Monoclonal Antibody (54 (RMT4-54)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

TIM-4

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

B430010N18Rik; RGD1564516; smuckler; soluble TIM 4; Spleen, mucin-containing, knockout of lymphotoxin protein; sTIM 4; T cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; Tim4; TIM-4; TIMD4; TIMD-4

Gene Symbols

TIMD4

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

TIMD4

Clone

54 (RMT4-54)

Applications

Flow Cytometry

Conjugate

PerCP-eFluor 710

Host Species

Rat

Target Species

Mouse

Gene Accession No.

Q6U7R4

Gene ID (Entrez)

276891

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The monoclonal antibody 54 (RMT4-54) recognizes mouse Tim-4 (T cell, Ig, mucin)
  • Unlike other Tim family members, Tim-4 is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages
  • Tim-4 has been shown to interact with Tim-1 thereby suggesting a role of Tim-4 in the balance of Th1/Th2 regulation
  • Additionally Tim-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells
  • Expression is found on a subset of CD11c+ splenic cells
  • Applications Reported: This 54 (RMT4-54) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 54 (RMT4-54) antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
  • We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
  • TIM-4 Monoclonal antibody specifically detects TIM-4 in Mouse samples
  • It is validated for Flow Cytometry.

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Antigen:
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Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
B430010N18Rik; RGD1564516; smuckler; soluble TIM 4; Spleen, mucin-containing, knockout of lymphotoxin protein; sTIM 4; T cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; Tim4; TIM-4; TIMD4; TIMD-4

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TIMD4

Primary or Secondary:
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Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
TIMD4

Clone:
54 (RMT4-54)

Applications:
Flow Cytometry

Conjugate:
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Host Species:
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Target Species:
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Gene Accession No.:
Q6U7R4

Gene ID (Entrez):
276891

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Primary or Secondary:
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