TIM-4 Monoclonal Antibody (54 (RMT4-54)), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 54 recognizes mouse Tim-4 (T cell, Ig, mucin)
• Unlike other Tim family members, Tim-4 is not expressed on T lymphocytes but is found on antigen presenting cells such as dendritic cells and macrophages
• Tim-4 has been shown to interact with Tim-1 thereby suggesting a role of Tim-4 in the balance of Th1/Th2 regulation
• Additionally Tim-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells
• Expression is found on a subset of CD11c+ splenic cells
• Applications Reported: This 54 (RMT4-54) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 54 (RMT4-54) antibody has been tested by flow cytometric analysis of resident peritoneal macrophages
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity
• TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production
• Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain
• The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.