FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined
• Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice
• Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells
• Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat
• 00-5523) and protocol reveals staining of the CD4+CD25^bright population
• The epitope from 236A/E7 is different from that of PCH101 (cat
• 72-5776)
• Applications Reported: This 236A/E7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat
• 00-5523) and protocol
• Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins)
• This can be used at 5 μL (0.25 μg) per test
• FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis
• FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells
• FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells
• Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice
• In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome
• Transcript variants of FOXP3 encoding different isoforms have been identified
• In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.