CD11b Monoclonal Antibody (M1/70), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM
• CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen
• Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity
• M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells
• Through interactions with its ligands, CD11b participates in adhesive cell interactions
• Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse splenocyte or bone marrow cell suspensions
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm)
• Most instruments will require a 685 LP mirror and 710/20 filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18)
• The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3)
• CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain
• CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions
• CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue
• The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages
• Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages
• Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.