7092323

CD11c Monoclonal Antibody (N418), eFluor™ 660, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD11c

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

AI449405; CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement component 3 receptor 4 subunit; complement receptor 4; Cr4; integrin alpha X; integrin alpha-X; integrin aX; integrin subunit alpha X; integrin, alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Itgax; Leu M5; leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; N418; p150 95 integrin alpha chain; RGD1561123; SLEB6

Gene Symbols

Itgax

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Itgax

Clone

N418

Applications

Flow Cytometry

Conjugate

eFluor 660

Host Species

Armenian Hamster

Target Species

Mouse

Gene Accession No.

Q9QXH4

Gene ID (Entrez)

16411

Isotype

IgG

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X
  • CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer
  • CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells
  • CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions
  • N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer
  • Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • eFluor™ 660 is a replacement for Alexa Fluor™ 647
  • eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD11, along with CD18, form a heterodimer adhesion molecule
  • In particular, CD11 is composed of CD11a, CD11b and CD11c
  • CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils
  • CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes
  • CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

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Antigen:
CD11c

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AI449405; CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement component 3 receptor 4 subunit; complement receptor 4; Cr4; integrin alpha X; integrin alpha-X; integrin aX; integrin subunit alpha X; integrin, alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Itgax; Leu M5; leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; N418; p150 95 integrin alpha chain; RGD1561123; SLEB6

Gene Symbols:
Itgax

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Itgax

Clone:
N418

Applications:
Flow Cytometry

Conjugate:
eFluor 660

Host Species:
Armenian Hamster

Target Species:
Mouse

Gene Accession No.:
Q9QXH4

Gene ID (Entrez):
16411

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
Mature Macrophage Marker

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
ED2-like antigen; Macrophages; Mature Macrophages

Gene Symbols:
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Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
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Clone:
eBio25F9 (25F9)

Applications:
Flow Cytometry

Conjugate:
eFluor 660

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
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Gene ID (Entrez):
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Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD324 (E-Cadherin)

Concentration:
0.2 mg/mL

Classification:
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Form:
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Regulatory Status:
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Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AA960649; ARC-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell adhesion molecule; cell-CAM 120/80; ECAD; E-cad; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Ecadherin; E-cadherin; E-cadherin 1; E-cadherin precursor; Epithelial cadherin; hab; half baked; LCAM; L-CAM; Um; UVO; Uvomorulin

Gene Symbols:
CDH1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CDH1

Clone:
DECMA-1

Applications:
Flow Cytometry, Immunocytochemistry

Conjugate:
eFluor 660

Host Species:
Rat

Target Species:
Canine, Human, Mouse

Gene Accession No.:
F1PAA9, P09803, P12830

Gene ID (Entrez):
12550, 442858, 999

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
Rat IgG1 kappa

Concentration:
0.2 mg/mL

Classification:
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Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1

Gene Symbols:
__

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
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Clone:
eBRG1

Applications:
Control, Flow Cytometry, Immunohistochemistry

Conjugate:
eFluor 660

Host Species:
Rat

Target Species:
Not Applicable

Gene Accession No.:
__

Gene ID (Entrez):
__

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
__