7092324

Mature Macrophage Marker Monoclonal Antibody (eBio25F9 (25F9)), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Mature Macrophage Marker

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

Clone

eBio25F9 (25F9)

Applications

Flow Cytometry

Conjugate

eFluor 660

Host Species

Mouse

Target Species

Human

Gene Alias

ED2-like antigen; Macrophages; Mature Macrophages

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

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Description

  • Description: The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures
  • Expression is thought to correlate with endocytic macrophages and is useful in many disease models
  • Expression is not found on immature macrophages or monocytes or any other hematopoietic cell
  • Applications Reported: This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This eBio25F9 (25F9) antibody has been pre-titrated and tested by flow cytometric analysis of cultured human macrosphages from monocytes
  • This can be used at 5 μL (0.25 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • eFluor® 660 is a replacement for Alexa Fluor® 647
  • eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • Mature Macrophage Marker Monoclonal antibody specifically detects Mature Macrophage Marker in Human samples
  • It is validated for Flow Cytometry.

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Show Difference

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7092324

Mouse Monoclonal Antibody...


Antigen:
Mature Macrophage Marker

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
eBio25F9 (25F9)

Applications:
Flow Cytometry

Conjugate:
eFluor 660

Host Species:
Mouse

Target Species:
Human

Gene Alias:
ED2-like antigen; Macrophages; Mature Macrophages

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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Antigen:
CD324 (E-Cadherin)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
DECMA-1

Applications:
Flow Cytometry, Immunocytochemistry

Conjugate:
eFluor 660

Host Species:
Rat

Target Species:
Canine, Human, Mouse

Gene Alias:
AA960649; ARC-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell adhesion molecule; cell-CAM 120/80; ECAD; E-cad; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Ecadherin; E-cadherin; E-cadherin 1; E-cadherin precursor; Epithelial cadherin; hab; half baked; LCAM; L-CAM; Um; UVO; Uvomorulin

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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Rat Isotype Control...


Antigen:
Rat IgG1 kappa

Concentration:
0.2 mg/mL

Classification:
__

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
__

Clone:
eBRG1

Applications:
Control, Flow Cytometry, Immunohistochemistry

Conjugate:
eFluor 660

Host Species:
Rat

Target Species:
Not Applicable

Gene Alias:
Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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Antigen:
EOMES

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
Dan11mag

Applications:
Flow Cytometry

Conjugate:
eFluor 660

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
C77258; EOMES; eomesodermin; eomesodermin homolog; eomesodermin homolog (Xenopus laevis); T-box brain protein 2; T-box transcription factor; TBR2; TBR-2; T-brain-2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!