7091479

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

SSEA4

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Isotype

IgG3

Purification Method

Affinity chromatography

Product Type

Antibody

Clone

eBioMC-813-70 (MC-813-70)

Applications

Flow Cytometry

Conjugate

Alexa Fluor 488

Host Species

Mouse

Target Species

Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit

Gene Alias

SSEA-4; stage-specific embryonic antigen 4

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

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Description

  • Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein
  • Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation
  • Recently expression has also been found on bone marrow mesenchymal stem cells
  • The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys
  • Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line
  • This can be used at 5 μL (1 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS)
  • In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation
  • Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

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Antigen:
SSEA4

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Isotype:
IgG3

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
eBioMC-813-70 (MC-813-70)

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 488

Host Species:
Mouse

Target Species:
Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit

Gene Alias:
SSEA-4; stage-specific embryonic antigen 4

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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Mouse Monoclonal Antibody...


Antigen:
PLZF

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
Mags.21F7

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 488

Host Species:
Mouse

Target Species:
Human, Mouse

Gene Alias:
AI467657; Green's luxoid; lu; luxoid; Lx; PLZF; Polydactyly-luxate syndrome; promyelocytic leukaemia zinc finger; promyelocytic leukemia zinc finger; Promyelocytic leukemia zinc finger protein; ZBTB16; Zfp145; zinc finger and BTB domain containing 16; zinc finger and BTB domain containing 16 protein; zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); zinc finger protein PLZF; ZNF145

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Primary

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Antigen:
IL-33R (ST2)

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
RMST2-2

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 488

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
DER4; Fit1; Fit-1; Fos-responsive gene 1 protein; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; IL1RL1; IL-1RL1; IL33R; IL-33R; interleukin 1 receptor like 1; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin1 receptor like 1; interleukin-1 receptor-like 1; Interleukin-33 receptor alpha chain; Ly84; lymphocyte antigen 84; Protein ST2; Protein T1; sIL 33R; soluble IL 33 Receptor; soluble IL 33R; St2; ST2L; St2-rs1; ST2V; Ste2; T1; T1/ST2

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Antigen:
Alpha-Smooth Muscle Actin

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Clone:
1A4

Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot

Conjugate:
Alexa Fluor 488

Host Species:
Mouse

Target Species:
Human, Mouse, Rat

Gene Alias:
0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; ASMA; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!