7092351

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

SSEA1

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias

FUTIV, Lewis X, SSEA-1

Gene Symbols

FUT4

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

FUT4

Clone

eBioMC-480 (MC-480)

Applications

Flow Cytometry

Conjugate

eFluor 660

Host Species

Mouse

Target Species

Human, Mouse

Gene Accession No.

P22083, Q11127

Gene ID (Entrez)

14345, 2526

Isotype

IgM

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG)
  • No immunoreactivity is evident with undifferentiated human EC and ES cells
  • Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished
  • Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes
  • SSEA-1 is associated with cell adhesion, migration and differentiation
  • Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of the F9 cell line
  • This can be used at 5 μL (0.06 μg μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • eFluor™ 660 is a replacement for Alexa Fluor™ 647
  • eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g
  • granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells
  • There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x
  • Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction
  • This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells
  • Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins
  • CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

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Show Difference

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7092351

Mouse Monoclonal Antibody...


Antigen:
SSEA1

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
FUTIV, Lewis X, SSEA-1

Gene Symbols:
FUT4

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
FUT4

Clone:
eBioMC-480 (MC-480)

Applications:
Flow Cytometry

Conjugate:
eFluor 660

Host Species:
Mouse

Target Species:
Human, Mouse

Gene Accession No.:
P22083, Q11127

Gene ID (Entrez):
14345, 2526

Isotype:
IgM

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD3

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3

Gene Symbols:
Cd247, Cd3d, CD3E, CD3g

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
__

Clone:
OKT3

Applications:
Flow Cytometry

Conjugate:
Alexa Fluor 488

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P04234, P07766, P09693, P20963

Gene ID (Entrez):
915, 916, 917, 919

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD324 (E-Cadherin)

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AA960649; ARC-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell adhesion molecule; cell-CAM 120/80; ECAD; E-cad; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Ecadherin; E-cadherin; E-cadherin 1; E-cadherin precursor; Epithelial cadherin; hab; half baked; LCAM; L-CAM; Um; UVO; Uvomorulin

Gene Symbols:
CDH1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CDH1

Clone:
DECMA-1

Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen)

Conjugate:
Alexa Fluor 488

Host Species:
Rat

Target Species:
Canine, Human, Mouse

Gene Accession No.:
F1PAA9, P09803, P12830

Gene ID (Entrez):
12550, 442858, 999

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
SOX2

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MCOPS3 (Microphthalmia Syndromic type 3); RGD1565646; sex determining region Y-box 2; SOX 2; SOX2; Sox-2; soxp; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box 2; SRY-box 2; SOX2; SRY-box containing gene 2; SRY-box transcription factor 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2; wu:fb83g04; wu:fc14d07; ysb; zgc:65860; zgc:77389

Gene Symbols:
SOX2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
SOX2

Clone:
Btjce

Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Conjugate:
Alexa Fluor 488

Host Species:
Rat

Target Species:
Human, Mouse

Gene Accession No.:
P48431, P48432

Gene ID (Entrez):
20674, 6657

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody