EOMES Monoclonal Antibody (WD1928), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2)
• Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates
• In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells
• Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R
• Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer Set (cat 00-5523) and protocol
• Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins)
• This can be used at 5 μL (0.03 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box
• T-box genes encode transcription factors involved in the regulation of developmental processes
• A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation
• TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.