7091478
SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
SSEA3
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Isotype
IgM
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
eBioMC-631 (MC-631)
Applications
Flow Cytometry
Conjugate
Alexa Fluor 488
Host Species
Rat
Target Species
Human, Mouse
Gene Alias
SSEA-3; stage-specific embryonic antigen 3
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
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Description
- Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3)
- Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation
- Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line
- This can be used at 5 μL (1 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
- Filtration: 0.2 μm post-manufacturing filtered
- SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS)
- In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation
- Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
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Antigen: SSEA3
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Isotype: IgM
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: eBioMC-631 (MC-631)
Applications: Flow Cytometry
Conjugate: Alexa Fluor 488
Host Species: Rat
Target Species: Human, Mouse
Gene Alias: SSEA-3; stage-specific embryonic antigen 3
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Antigen:
SSEA3
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Isotype:
IgM
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
eBioMC-631 (MC-631)
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 488
Host Species:
Rat
Target Species:
Human, Mouse
Gene Alias:
SSEA-3; stage-specific embryonic antigen 3
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Antigen: SSEA4
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Isotype: IgG3
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: eBioMC-813-70 (MC-813-70)
Applications: Flow Cytometry
Conjugate: Alexa Fluor 488
Host Species: Mouse
Target Species: Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit
Gene Alias: SSEA-4; stage-specific embryonic antigen 4
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Antigen:
SSEA4
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Isotype:
IgG3
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
eBioMC-813-70 (MC-813-70)
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 488
Host Species:
Mouse
Target Species:
Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit
Gene Alias:
SSEA-4; stage-specific embryonic antigen 4
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Antigen: PLZF
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: Mags.21F7
Applications: Flow Cytometry
Conjugate: Alexa Fluor 488
Host Species: Mouse
Target Species: Human, Mouse
Gene Alias: AI467657; Green's luxoid; lu; luxoid; Lx; PLZF; Polydactyly-luxate syndrome; promyelocytic leukaemia zinc finger; promyelocytic leukemia zinc finger; Promyelocytic leukemia zinc finger protein; ZBTB16; Zfp145; zinc finger and BTB domain containing 16; zinc finger and BTB domain containing 16 protein; zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); zinc finger protein PLZF; ZNF145
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Antigen:
PLZF
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
Mags.21F7
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 488
Host Species:
Mouse
Target Species:
Human, Mouse
Gene Alias:
AI467657; Green's luxoid; lu; luxoid; Lx; PLZF; Polydactyly-luxate syndrome; promyelocytic leukaemia zinc finger; promyelocytic leukemia zinc finger; Promyelocytic leukemia zinc finger protein; ZBTB16; Zfp145; zinc finger and BTB domain containing 16; zinc finger and BTB domain containing 16 protein; zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); zinc finger protein PLZF; ZNF145
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Antigen: IL-33R (ST2)
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Clone: RMST2-2
Applications: Flow Cytometry
Conjugate: Alexa Fluor 488
Host Species: Rat
Target Species: Mouse
Gene Alias: DER4; Fit1; Fit-1; Fos-responsive gene 1 protein; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; IL1RL1; IL-1RL1; IL33R; IL-33R; interleukin 1 receptor like 1; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin1 receptor like 1; interleukin-1 receptor-like 1; Interleukin-33 receptor alpha chain; Ly84; lymphocyte antigen 84; Protein ST2; Protein T1; sIL 33R; soluble IL 33 Receptor; soluble IL 33R; St2; ST2L; St2-rs1; ST2V; Ste2; T1; T1/ST2
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Antigen:
IL-33R (ST2)
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
RMST2-2
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 488
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
DER4; Fit1; Fit-1; Fos-responsive gene 1 protein; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; IL1RL1; IL-1RL1; IL33R; IL-33R; interleukin 1 receptor like 1; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin1 receptor like 1; interleukin-1 receptor-like 1; Interleukin-33 receptor alpha chain; Ly84; lymphocyte antigen 84; Protein ST2; Protein T1; sIL 33R; soluble IL 33 Receptor; soluble IL 33R; St2; ST2L; St2-rs1; ST2V; Ste2; T1; T1/ST2
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!