7091538
CD45.2 Monoclonal Antibody (104), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD45.2
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
Gene Symbols
Ptprc
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Ptprc
Clone
104
Applications
Flow Cytometry
Conjugate
Alexa Fluor 700
Host Species
Mouse
Target Species
Mouse
Gene Accession No.
P06800
Gene ID (Entrez)
19264
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains
- The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR
- CD45.2 is expressed by all leukocytes in these strains
- Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm)
- Most instruments will require a 685 LP mirror and 710/20 filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
- Filtration: 0.2 μm post-manufacturing filtered
- CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
- CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
- CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains
- These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
- Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
- CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Compare Similar Items
Show Difference
Antigen: CD45.2
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
Gene Symbols: Ptprc
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Ptprc
Clone: 104
Applications: Flow Cytometry
Conjugate: Alexa Fluor 700
Host Species: Mouse
Target Species: Mouse
Gene Accession No.: P06800
Gene ID (Entrez): 19264
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD45.2
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
Gene Symbols:
Ptprc
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Ptprc
Clone:
104
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 700
Host Species:
Mouse
Target Species:
Mouse
Gene Accession No.:
P06800
Gene ID (Entrez):
19264
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD127
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: CD127; CD127 antigen; CDW127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R a; IL 7Ra; IL 7Ra; IL7 receptor; IL-7 receptor alpha chain; IL7 receptor subunit alpha; IL-7 receptor subunit alpha; IL7R; IL-7R; IL7R a; IL7R alpha; IL7R subunit alpha; IL-7R subunit alpha; IL7R a; IL7RA; IL-7RA; IL-7Ralpha; IL-7R-alpha; IL7Ra; ILRA; interleukin 7 receptor; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin7 receptor subunit alpha; interleukin-7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R
Gene Symbols: Il7r
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Il7r
Clone: A7R34
Applications: Flow Cytometry
Conjugate: Alexa Fluor 700
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P16872
Gene ID (Entrez): 16197
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD127
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
CD127; CD127 antigen; CDW127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R a; IL 7Ra; IL 7Ra; IL7 receptor; IL-7 receptor alpha chain; IL7 receptor subunit alpha; IL-7 receptor subunit alpha; IL7R; IL-7R; IL7R a; IL7R alpha; IL7R subunit alpha; IL-7R subunit alpha; IL7R a; IL7RA; IL-7RA; IL-7Ralpha; IL-7R-alpha; IL7Ra; ILRA; interleukin 7 receptor; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin7 receptor subunit alpha; interleukin-7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R
Gene Symbols:
Il7r
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Il7r
Clone:
A7R34
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 700
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P16872
Gene ID (Entrez):
16197
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: MHC Class II (I-A/I-E)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
Gene Symbols: H2-Ab1, H2-Eb1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: H2-Ab1
Clone: M5/114.15.2
Applications: Flow Cytometry
Conjugate: Alexa Fluor 700
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P01921, P04230, P06342, P06343, P06344, P06345, P06346, P14483
Gene ID (Entrez): 14961, 14969
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
MHC Class II (I-A/I-E)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
Gene Symbols:
H2-Ab1, H2-Eb1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
H2-Ab1
Clone:
M5/114.15.2
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 700
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P01921, P04230, P06342, P06343, P06344, P06345, P06346, P14483
Gene ID (Entrez):
14961, 14969
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Ki-67
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
Gene Symbols: Mki67
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Mki67
Clone: SolA15
Applications: Flow Cytometry
Conjugate: Alexa Fluor 700
Host Species: Rat
Target Species: Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat
Gene Accession No.: E9PVX6, P46013
Gene ID (Entrez): 100686578, 102135895, 17345, 291234, 4288
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Ki-67
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
Gene Symbols:
Mki67
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Mki67
Clone:
SolA15
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 700
Host Species:
Rat
Target Species:
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat
Gene Accession No.:
E9PVX6, P46013
Gene ID (Entrez):
100686578, 102135895, 17345, 291234, 4288
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody