7092289

Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor™ 780, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Ly-6C

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

AA682074; AA959465; Ly6c; Ly-6C; Ly6c1; Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C

Gene Symbols

Ly6c1

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Ly6c1

Clone

HK1.4

Applications

Flow Cytometry

Conjugate

APC-eFluor 780

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P0CW02

Gene ID (Entrez)

17067

Isotype

IgG2c κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family
  • Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes
  • Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes
  • These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells
  • In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release
  • Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm
  • They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments
  • In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes
  • Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

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Antigen:
Ly-6C

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AA682074; AA959465; Ly6c; Ly-6C; Ly6c1; Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C

Gene Symbols:
Ly6c1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Ly6c1

Clone:
HK1.4

Applications:
Flow Cytometry

Conjugate:
APC-eFluor 780

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P0CW02

Gene ID (Entrez):
17067

Isotype:
IgG2c κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD3

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3

Gene Symbols:
Cd247, Cd3d, CD3E, CD3g

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
__

Clone:
17A2

Applications:
Flow Cytometry

Conjugate:
eFluor 450

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P04235, P11942, P22646, P24161

Gene ID (Entrez):
12500, 12501, 12502, 12503

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD11b

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product

Gene Symbols:
ITGAM

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
ITGAM

Clone:
M1/70

Applications:
Flow Cytometry

Conjugate:
eFluor 450

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P05555

Gene ID (Entrez):
16409

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7092295

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Antigen:
Rat IgG2b kappa

Concentration:
0.2 mg/mL

Classification:
__

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Gene Symbols:
__

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
__

Clone:
eB149/10H5

Applications:
Control, Flow Cytometry

Conjugate:
eFluor 450

Host Species:
Rat

Target Species:
Not Applicable

Gene Accession No.:
__

Gene ID (Entrez):
__

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
__