7092373
Gata-3 Monoclonal Antibody (TWAJ), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Gata-3
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Gene Symbols
GATA3
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
GATA3
Clone
TWAJ
Applications
Flow Cytometry, Immunocytochemistry
Conjugate
Alexa Fluor 488
Host Species
Rat
Target Species
Human, Mouse, Porcine, Rhesus Monkey
Gene Accession No.
P23771, P23772
Gene ID (Entrez)
14462, 2625, 713840, 733631
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors
- Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation
- Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney
- During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal
- The Gata-3 is also essential for T cell commitment and survival
- In the thymus, expression is found mainly on the CD4 single positive cells
- During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation
- Alternative splice variants have been reported especially in the MCF7 cell line
- The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein
- Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(cat 00-5523) Crossreactivity in rhesus monkeys has been published
- Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat
- 00-5523) and protocol
- The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins
- Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes
- GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain
- GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages
- GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers
- Labastie et al
- (1994) cloned the human gene and the 5-prime end of the mouse gene
- The human gene comprises 6 exons distributed over 17 kb of DNA
- Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
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Antigen: Gata-3
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Gene Symbols: GATA3
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: GATA3
Clone: TWAJ
Applications: Flow Cytometry, Immunocytochemistry
Conjugate: Alexa Fluor 488
Host Species: Rat
Target Species: Human, Mouse, Porcine, Rhesus Monkey
Gene Accession No.: P23771, P23772
Gene ID (Entrez): 14462, 2625, 713840, 733631
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Gata-3
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Gene Symbols:
GATA3
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
GATA3
Clone:
TWAJ
Applications:
Flow Cytometry, Immunocytochemistry
Conjugate:
Alexa Fluor 488
Host Species:
Rat
Target Species:
Human, Mouse, Porcine, Rhesus Monkey
Gene Accession No.:
P23771, P23772
Gene ID (Entrez):
14462, 2625, 713840, 733631
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD3
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3
Gene Symbols: Cd247, Cd3d, CD3E, CD3g
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: __
Clone: 17A2
Applications: Flow Cytometry
Conjugate: Alexa Fluor 700
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P04235, P11942, P22646, P24161
Gene ID (Entrez): 12500, 12501, 12502, 12503
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD3
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3
Gene Symbols:
Cd247, Cd3d, CD3E, CD3g
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
__
Clone:
17A2
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 700
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P04235, P11942, P22646, P24161
Gene ID (Entrez):
12500, 12501, 12502, 12503
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: αβ T-Cell Receptor
Concentration: 0.5mg/mL
Classification: Monoclonal
Form: __
Regulatory Status: RUO
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias: __
Gene Symbols: __
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Gene: __
Clone: R73
Applications: Flow Cytometry
Conjugate: FITC
Host Species: Mouse
Target Species: Rat
Gene Accession No.: __
Gene ID (Entrez): __
Isotype: IgG1 κ
Purification Method: Affinity Purified
Product Type: __
Antigen:
αβ T-Cell Receptor
Concentration:
0.5mg/mL
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias:
__
Gene Symbols:
__
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Gene:
__
Clone:
R73
Applications:
Flow Cytometry
Conjugate:
FITC
Host Species:
Mouse
Target Species:
Rat
Gene Accession No.:
__
Gene ID (Entrez):
__
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Product Type:
__
Antigen: αβ T-Cell Receptor
Concentration: 0.5mg/mL
Classification: Monoclonal
Form: __
Regulatory Status: RUO
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias: __
Gene Symbols: __
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Gene: __
Clone: R73
Applications: Flow Cytometry
Conjugate: FITC
Host Species: Mouse
Target Species: Rat
Gene Accession No.: __
Gene ID (Entrez): __
Isotype: IgG1 κ
Purification Method: Affinity Purified
Product Type: __
Antigen:
αβ T-Cell Receptor
Concentration:
0.5mg/mL
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias:
__
Gene Symbols:
__
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Gene:
__
Clone:
R73
Applications:
Flow Cytometry
Conjugate:
FITC
Host Species:
Mouse
Target Species:
Rat
Gene Accession No.:
__
Gene ID (Entrez):
__
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Product Type:
__