7105385
PMEL17/SILV Mouse, Clone: SPM142, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
PMEL17/SILV
Dilution
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols
PMEL
Isotype
IgG1 κ
Purification Method
Protein A purified
Test Specificity
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma.
Clone
SPM142
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human, Canine (Negative), Rat (Negative)
Gene Accession No.
P40967
Gene ID (Entrez)
6490
Immunogen
Extract of pigmented melanoma metastases from lymph nodes
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Related Products
Description
- PMEL17/SILV Monoclonal specifically detects PMEL17/SILV in Human, Canine (Negative), Rat (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: PMEL17/SILV
Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols: PMEL
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma.
Clone: SPM142
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Canine (Negative), Rat (Negative)
Gene Accession No.: P40967
Gene ID (Entrez): 6490
Immunogen: Extract of pigmented melanoma metastases from lymph nodes
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Antigen:
PMEL17/SILV
Dilution:
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols:
PMEL
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma.
Clone:
SPM142
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Canine (Negative), Rat (Negative)
Gene Accession No.:
P40967
Gene ID (Entrez):
6490
Immunogen:
Extract of pigmented melanoma metastases from lymph nodes
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Antigen: PMEL17/SILV
Dilution: Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols: PMEL
Isotype: IgG2b κ
Purification Method: Protein A purified
Test Specificity: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Clone: SPM286
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Equine, Rat (Negative)
Gene Accession No.: P40967
Gene ID (Entrez): 6490
Immunogen: Membranes from a human melanoma metastasis
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Antigen:
PMEL17/SILV
Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols:
PMEL
Isotype:
IgG2b κ
Purification Method:
Protein A purified
Test Specificity:
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Clone:
SPM286
Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Equine, Rat (Negative)
Gene Accession No.:
P40967
Gene ID (Entrez):
6490
Immunogen:
Membranes from a human melanoma metastasis
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Antigen: PMEL17/SILV
Dilution: Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols: PMEL
Isotype: IgG2b κ
Purification Method: Protein A purified
Test Specificity: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Clone: SPM286
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Equine, Rat (Negative)
Gene Accession No.: P40967
Gene ID (Entrez): 6490
Immunogen: Membranes from a human melanoma metastasis
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Antigen:
PMEL17/SILV
Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols:
PMEL
Isotype:
IgG2b κ
Purification Method:
Protein A purified
Test Specificity:
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Clone:
SPM286
Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Equine, Rat (Negative)
Gene Accession No.:
P40967
Gene ID (Entrez):
6490
Immunogen:
Membranes from a human melanoma metastasis
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Antigen: CCL16/HCC-4/LEC
Dilution: ELISA, Functional, SDS-Page
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: C-C motif chemokine 16, chemokine (C-C motif) ligand 16, Chemokine CC-4, Chemokine LEC, CKb12, HCC-4Liver-expressed chemokine, LCC-1Lymphocyte and monocyte chemoattractant, LEC, liver CC chemokine-1, LMCSmall-inducible cytokine A16, monotactin-1, MTN-1, NCC4IL-10-inducible chemokine, NCC-4ILINCK, new CC chemokine 4, SCYA16MGC117051, SCYL4, small inducible cytokine subfamily A (Cys-Cys), member 16
Gene Symbols: CCL16
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelo-suppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones. The ability of HCC-4 to markedly improve recognition of poorly immunogenic cells suggests that it could be an effective component of antitumor vaccines.
Clone: LEC66
Applications: ELISA, Functional Assay, SDS-Page
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: O15467
Gene ID (Entrez): 6360
Immunogen: Recombinant human HCC-4 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Antigen:
CCL16/HCC-4/LEC
Dilution:
ELISA, Functional, SDS-Page
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
C-C motif chemokine 16, chemokine (C-C motif) ligand 16, Chemokine CC-4, Chemokine LEC, CKb12, HCC-4Liver-expressed chemokine, LCC-1Lymphocyte and monocyte chemoattractant, LEC, liver CC chemokine-1, LMCSmall-inducible cytokine A16, monotactin-1, MTN-1, NCC4IL-10-inducible chemokine, NCC-4ILINCK, new CC chemokine 4, SCYA16MGC117051, SCYL4, small inducible cytokine subfamily A (Cys-Cys), member 16
Gene Symbols:
CCL16
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelo-suppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones. The ability of HCC-4 to markedly improve recognition of poorly immunogenic cells suggests that it could be an effective component of antitumor vaccines.
Clone:
LEC66
Applications:
ELISA, Functional Assay, SDS-Page
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
O15467
Gene ID (Entrez):
6360
Immunogen:
Recombinant human HCC-4 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.