7105469

Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (SPM552), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Chorionic Gonadotropin alpha Chain (HCG alpha)

Dilution

Western Blot 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

CGA glycoprotein hormones, alpha polypeptide, CG-ALPHA, FSHA, GPHa, GPHA1, HCG, LHA, TSHA

Gene Symbols

CGA

Isotype

IgG1 κ

Purification Method

Protein A purified

Test Specificity

This MAb reacts with a protein of ∼13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the ? and ? subunits. The subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).

Clone

SPM552

Applications

Western Blot, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human

Gene Accession No.

P01215

Gene ID (Entrez)

1081

Immunogen

Recombinant hCG alpha protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

13 kDa

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Description

  • Chorionic Gonadotropin alpha Chain (hCG alpha) Monoclonal specifically detects Chorionic Gonadotropin alpha Chain (hCG alpha) in Human samples
  • It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

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Antigen:
Chorionic Gonadotropin alpha Chain (HCG alpha)

Dilution:
Western Blot 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CGA glycoprotein hormones, alpha polypeptide, CG-ALPHA, FSHA, GPHa, GPHA1, HCG, LHA, TSHA

Gene Symbols:
CGA

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This MAb reacts with a protein of ∼13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the ? and ? subunits. The subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).

Clone:
SPM552

Applications:
Western Blot, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P01215

Gene ID (Entrez):
1081

Immunogen:
Recombinant hCG alpha protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
13 kDa

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Antigen:
HSP60

Dilution:
Western Blot 0.25-0.5ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), Heat shock protein 60, heat shock protein 65, HLD4, Hsp60, HSP-60, HSP60SPG13, HSP65, HuCHA60, Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)

Gene Symbols:
HSPD1

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Clone:
LK1

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Hamster, Primate, Rabbit, Sheep, Xenopus species

Gene Accession No.:
P10809

Gene ID (Entrez):
3329

Immunogen:
Recombinant human HSP60 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
60 kDa

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Antigen:
Insulin

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin

Gene Symbols:
INS

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Clone:
SPM139

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human, Porcine, Bovine, Mouse (Negative), Rat (Negative)

Gene Accession No.:
P01308

Gene ID (Entrez):
3630

Immunogen:
Full length (1-84 amino acid) purified pig insulin, conjugated to KLH

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
6 kDa

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Antigen:
Melan-A/MART-1

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A

Gene Symbols:
MLANA

Isotype:
IgG2b κ

Purification Method:
Protein A purified

Test Specificity:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Clone:
SPM342

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human, Equine, Mouse (Negative), Rat (Negative)

Gene Accession No.:
Q16655

Gene ID (Entrez):
2315

Immunogen:
Recombinant hMART-1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
__