7105619

Bcl-2 Mouse, Clone: SPM530, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Bcl-2

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.1-1ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse (Negative), Rat (Negative)

Gene Accession No.

P10415

Gene ID (Entrez)

596

Immunogen

A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

25 kDa

Clone

SPM530

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Apoptosis, Autophagy, Cancer, Cellular Markers, Tumor Suppressors

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

Gene Symbols

BCL2

Isotype

IgG1 κ

Purification Method

Protein A purified

Test Specificity

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

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Description

  • Bcl-2 Monoclonal specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Show Difference

Img

Fischer Scientific

7105619

Mouse Monoclonal Antibody...


Antigen:
Bcl-2

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.1-1ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse (Negative), Rat (Negative)

Gene Accession No.:
P10415

Gene ID (Entrez):
596

Immunogen:
A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
25 kDa

Clone:
SPM530

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Apoptosis, Autophagy, Cancer, Cellular Markers, Tumor Suppressors

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

Gene Symbols:
BCL2

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

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7105622

Mouse Monoclonal Antibody...


Antigen:
Bcl-2

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse (Negative), Rat (Negative)

Gene Accession No.:
P10415

Gene ID (Entrez):
596

Immunogen:
A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
25 kDa

Clone:
124

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Apoptosis, Autophagy, Cancer, Cellular Markers, Tumor Suppressors

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

Gene Symbols:
BCL2

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Img

Fischer Scientific

7105622

Mouse Monoclonal Antibody...


Antigen:
Bcl-2

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse (Negative), Rat (Negative)

Gene Accession No.:
P10415

Gene ID (Entrez):
596

Immunogen:
A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
25 kDa

Clone:
124

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Apoptosis, Autophagy, Cancer, Cellular Markers, Tumor Suppressors

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

Gene Symbols:
BCL2

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Img

Fischer Scientific

7105623

Mouse Monoclonal Antibody...


Antigen:
Bcl-2

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse (Negative), Rat (Negative)

Gene Accession No.:
P10415

Gene ID (Entrez):
596

Immunogen:
A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
25 kDa

Clone:
100/D5+124

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Apoptosis, Autophagy, Cancer, Cellular Markers, Tumor Suppressors

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

Gene Symbols:
BCL2

Isotype:
IgG

Purification Method:
Protein A purified

Test Specificity:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.