Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Bcl-2
1.0 mg/mL
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Unconjugated
Mouse
Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Oncogenes, Tumor Suppressors
PBS with No Preservative
596
Recombinant full-length human bcl-2 protein
Primary
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
25.5 kDa
BCL2/782
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Monoclonal
Purified
RUO
Human
apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
BCL2
IgG1 κ
Protein A or G purified
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.