7105522

IgM Antibody (IM260), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

IgM

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Accession No.

P01871

Gene ID (Entrez)

3507

Immunogen

Recombinant heavy chain of human IgM

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

75 kDa

Clone

IM260

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Immunology

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbols

IGHM

Isotype

IgG1 κ

Purification Method

Protein A purified

Test Specificity

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

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Description

  • IgM Monoclonal specifically detects IgM in Human samples
  • It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

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Antigen:
IgM

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P01871

Gene ID (Entrez):
3507

Immunogen:
Recombinant heavy chain of human IgM

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
75 kDa

Clone:
IM260

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Immunology

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbols:
IGHM

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

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7105524

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Antigen:
Kappa Light Chain

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P01601

Gene ID (Entrez):
3514

Immunogen:
Recombinant human Ig kappa chain

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
22.5 kDa

Clone:
KLC264

Applications:
Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Immunology

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbols:
IGKC

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

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7105525

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Antigen:
Kappa Light Chain

Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 0.5-1ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P01601

Gene ID (Entrez):
3514

Immunogen:
Human IgG-kappa myeloma protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
22.5 kDa

Clone:
TB28-2

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Immunology

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbols:
IGKC

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

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7105526

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Antigen:
Kappa Light Chain

Dilution:
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-2.0ug/million cells, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P01601

Gene ID (Entrez):
3514

Immunogen:
Purified human Ig kappa chain

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
22.5 kDa

Clone:
HP6053

Applications:
Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Immunology

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbols:
IGKC

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.