7105948
CD55/DAF Antibody (F4-29D9) - Azide and BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD55/DAF
Concentration
1.0 mg/mL
Applications
Flow Cytometry, Immunofluorescence, CyTOF
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Immunology
Formulation
PBS with No Preservative
Gene ID (Entrez)
1604
Immunogen
Human umbilical vein endothelial cells (HUVEC)
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
70 kDa
Clone
F4-29D9
Dilution
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC
Gene Symbols
CD55
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Description
- CD55/DAF Monoclonal specifically detects CD55/DAF in Human samples
- It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, CyTOF-ready.
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Antigen: CD55/DAF
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Immunology
Formulation: PBS with No Preservative
Gene ID (Entrez): 1604
Immunogen: Human umbilical vein endothelial cells (HUVEC)
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 70 kDa
Clone: F4-29D9
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC
Gene Symbols: CD55
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Antigen:
CD55/DAF
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
PBS with No Preservative
Gene ID (Entrez):
1604
Immunogen:
Human umbilical vein endothelial cells (HUVEC)
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
70 kDa
Clone:
F4-29D9
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC
Gene Symbols:
CD55
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Antigen: CD55/DAF
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Immunology
Formulation: PBS with No Preservative
Gene ID (Entrez): 1604
Immunogen: PHA stimulated human PBL
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 70 kDa
Clone: 143-30
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC
Gene Symbols: CD55
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Antigen:
CD55/DAF
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
PBS with No Preservative
Gene ID (Entrez):
1604
Immunogen:
PHA stimulated human PBL
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
70 kDa
Clone:
143-30
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC
Gene Symbols:
CD55
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Antigen: NGF R/TNFRSF16/p75NTR
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Formulation: PBS with No Preservative
Gene ID (Entrez): 4804
Immunogen: NGFR from A875 melanoma cells
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 75 kDa
Clone: NGFR5
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Baboon, Feline, Ferret, Monkey, Rabbit, Mouse (Negative), Rat (Negative)
Gene Alias: CD271, CD271 antigen, Gp80-LNGFR, member 16, nerve growth factor receptor, NGF receptor, p75 ICD, TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16), tumor necrosis factor receptor superfamily member 16
Gene Symbols: NGFR
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
Antigen:
NGF R/TNFRSF16/p75NTR
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Formulation:
PBS with No Preservative
Gene ID (Entrez):
4804
Immunogen:
NGFR from A875 melanoma cells
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
75 kDa
Clone:
NGFR5
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Baboon, Feline, Ferret, Monkey, Rabbit, Mouse (Negative), Rat (Negative)
Gene Alias:
CD271, CD271 antigen, Gp80-LNGFR, member 16, nerve growth factor receptor, NGF receptor, p75 ICD, TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16), tumor necrosis factor receptor superfamily member 16
Gene Symbols:
NGFR
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
Antigen: NF-L
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience
Formulation: PBS with No Preservative
Gene ID (Entrez): 4747
Immunogen: Crude neurofilament preparation from porcine spinal cord
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 68 kDa
Clone: NR-4
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat, Porcine, Chicken, Primate
Gene Alias: 68 kDa neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, Neurofilament triplet L protein, neurofilament, light polypeptide, neurofilament-light, NF68FLJ53642, NF-L, NFLlight polypeptide 68kDa
Gene Symbols: NEFL
Isotype: IgG1
Purification Method: Protein A or G purified
Test Specificity: This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Antigen:
NF-L
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience
Formulation:
PBS with No Preservative
Gene ID (Entrez):
4747
Immunogen:
Crude neurofilament preparation from porcine spinal cord
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
68 kDa
Clone:
NR-4
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Rat, Porcine, Chicken, Primate
Gene Alias:
68 kDa neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, Neurofilament triplet L protein, neurofilament, light polypeptide, neurofilament-light, NF68FLJ53642, NF-L, NFLlight polypeptide 68kDa
Gene Symbols:
NEFL
Isotype:
IgG1
Purification Method:
Protein A or G purified
Test Specificity:
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.