7106167
GFP Antibody (9F9.F9), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 44 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
GFP
Concentration
1 mg/ml
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunoblot, In vitro Assay, Dot Blot, KnockDown
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cellular Markers, Epitope Tags, Fusion Proteins, Mitochondrial Fusion Proteins, Mitochondrial Markers
Gene Alias
clone 9F9.F9, Enhanced Green Fluorescent Protein, GFP monoclonal, Green Fluorescent Protein, green fluorescent protein (gfp)
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Primary or Secondary
Primary
Content And Storage
Store at -20°C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
27 kDa
Clone
9F9.F9
Dilution
Western Blot 1:3000-1:30000, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:10000-1:30000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting, In vitro assay, Dot Blot 1:100 - 1:2000, Knockdown Validated
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Jellyfish
Gene Symbols
GFP
Isotype
IgG1 κ
Purification Method
Protein A purified
Test Specificity
Reactivity is observed against recombinant Green Fluorescent Protein from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
Related Products
Description
- GFP Monoclonal specifically detects GFP in Non-species specific samples
- It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunoblotting, In vitro assay, Dot Blot, Knockdown Validated.
Compare Similar Items
Show Difference
Antigen: GFP
Concentration: 1 mg/ml
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunoblot, In vitro Assay, Dot Blot, KnockDown
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cellular Markers, Epitope Tags, Fusion Proteins, Mitochondrial Fusion Proteins, Mitochondrial Markers
Gene Alias: clone 9F9.F9, Enhanced Green Fluorescent Protein, GFP monoclonal, Green Fluorescent Protein, green fluorescent protein (gfp)
Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Primary or Secondary: Primary
Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 27 kDa
Clone: 9F9.F9
Dilution: Western Blot 1:3000-1:30000, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:10000-1:30000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting, In vitro assay, Dot Blot 1:100 - 1:2000, Knockdown Validated
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Jellyfish
Gene Symbols: GFP
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Reactivity is observed against recombinant Green Fluorescent Protein from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
Antigen:
GFP
Concentration:
1 mg/ml
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunoblot, In vitro Assay, Dot Blot, KnockDown
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cellular Markers, Epitope Tags, Fusion Proteins, Mitochondrial Fusion Proteins, Mitochondrial Markers
Gene Alias:
clone 9F9.F9, Enhanced Green Fluorescent Protein, GFP monoclonal, Green Fluorescent Protein, green fluorescent protein (gfp)
Immunogen:
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Primary or Secondary:
Primary
Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
27 kDa
Clone:
9F9.F9
Dilution:
Western Blot 1:3000-1:30000, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:10000-1:30000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting, In vitro assay, Dot Blot 1:100 - 1:2000, Knockdown Validated
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Jellyfish
Gene Symbols:
GFP
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Reactivity is observed against recombinant Green Fluorescent Protein from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
Antigen: GLI-2
Concentration: 1 mg/ml
Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Cancer, Stem Cell Signaling Pathway
Gene Alias: GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2
Immunogen: This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)
Primary or Secondary: Primary
Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 167.78 kDa
Clone: __
Dilution: Western Blot 1:500-1:2000, ELISA 1:10000-1:30000, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Human, Mouse, Rat
Gene Symbols: GLI2
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.
Antigen:
GLI-2
Concentration:
1 mg/ml
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Cancer, Stem Cell Signaling Pathway
Gene Alias:
GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2
Immunogen:
This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)
Primary or Secondary:
Primary
Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
167.78 kDa
Clone:
__
Dilution:
Western Blot 1:500-1:2000, ELISA 1:10000-1:30000, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat
Gene Symbols:
GLI2
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.
Antigen: Nanog
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Embryonic Stem Cell Markers, Stem Cell Markers
Gene Alias: FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Immunogen: Recombinant human Nanog (1-154aa) purified from E. coli (NP_079141).
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 35 kDa
Clone: 5A10
Dilution: Western Blot 1:500-1:1000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:100
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Symbols: NANOG
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
Antigen:
Nanog
Concentration:
1.0 mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Embryonic Stem Cell Markers, Stem Cell Markers
Gene Alias:
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Immunogen:
Recombinant human Nanog (1-154aa) purified from E. coli (NP_079141).
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen:
35 kDa
Clone:
5A10
Dilution:
Western Blot 1:500-1:1000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:100
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Symbols:
NANOG
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
Antigen: Collagen III alpha 1/COL3A1
Concentration: 1.0 mg/mL
Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: __
Gene Alias: alpha1 (III) collagen, Collagen 3, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, Collagen-3, EDS4A, Ehlers-Danlos syndrome type IV, autosomal dominant, FLJ34534
Immunogen: __
Primary or Secondary: Primary
Content And Storage: Store at 4C in the dark.
Molecular Weight of Antigen: __
Clone: 1E7-D7/Col3
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat, Canine, Kangaroo, Rabbit
Gene Symbols: COL3A1
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Highly specific for type III collagen. It has been shown to have no cross reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Antigen:
Collagen III alpha 1/COL3A1
Concentration:
1.0 mg/mL
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Gene Alias:
alpha1 (III) collagen, Collagen 3, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, Collagen-3, EDS4A, Ehlers-Danlos syndrome type IV, autosomal dominant, FLJ34534
Immunogen:
__
Primary or Secondary:
Primary
Content And Storage:
Store at 4C in the dark.
Molecular Weight of Antigen:
__
Clone:
1E7-D7/Col3
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Rat, Canine, Kangaroo, Rabbit
Gene Symbols:
COL3A1
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Highly specific for type III collagen. It has been shown to have no cross reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).