CD11c Monoclonal Antibody (N418), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX
• CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer
• CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells
• CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions
• N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer
• Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• CD11, along with CD18, form a heterodimer adhesion molecule
• In particular, CD11 is composed of CD11a, CD11b and CD11c
• CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils
• CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes
• CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.