CD11b Monoclonal Antibody (M1/70), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain
• CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer
• This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes
• CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions
• Applications Reported: This 3.9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3.9 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells of normal human peripheral blood cells
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
• We recommend using a 710/50 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• SB-4400) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18)
• The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3)
• CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain
• CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions
• CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue
• The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages
• Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages
• Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.