Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R), Novus Biologicals™
Rabbit Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Biotin (Vitamin B7 or Vitamin H)
Dilution
Western Blot, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
Classification
Monoclonal
Form
Purified
Target Species
All species
Immunogen
Biotinylated sheep immunoglobulin
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
2.4 kDa
Clone
BTN/2032R
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
Conjugate
Unconjugated
Host Species
Mouse
Formulation
Prepared in 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
Isotype
IgG1
Purification Method
Protein A or G purified
Test Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Related Products
Description
- Biotin (Vitamin B7 or Vitamin H) Monoclonal specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization.
Compare Similar Items
Show Difference
Antigen: Biotin (Vitamin B7 or Vitamin H)
Dilution: Western Blot, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
Classification: Monoclonal
Form: Purified
Target Species: All species
Immunogen: Biotinylated sheep immunoglobulin
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 2.4 kDa
Clone: BTN/2032R
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
Conjugate: Unconjugated
Host Species: Mouse
Formulation: Prepared in 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
Isotype: IgG1
Purification Method: Protein A or G purified
Test Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Antigen: Biotin (Vitamin B7 or Vitamin H)
Dilution: Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
Classification: Monoclonal
Form: Purified
Target Species: All species
Immunogen: Biotinylated sheep immunoglobulin
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 2.4 kDa
Clone: BTN/2032R
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
Conjugate: Unconjugated
Host Species: Mouse
Formulation: 10mM PBS - BSA free with No Preservative
Isotype: IgG1
Purification Method: Protein A or G purified
Test Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Antigen: TYRP1
Dilution: ELISA 2-4 ug/ml, Immunohistochemistry-Paraffin 1-2 ug/ml, SDS-Page
Classification: Monoclonal
Form: Purified
Target Species: Human
Immunogen: Recombinant fragment of human TYRP1 protein (around aa 257-377) (exact sequence is proprietary)
Primary or Secondary: Primary
Content And Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 75 kDa
Clone: TYRP1/1986
Applications: ELISA, Immunohistochemistry (Paraffin), SDS-Page
Conjugate: Unconjugated
Host Species: Mouse
Formulation: 10mM PBS - BSA free with No Preservative
Isotype: __
Purification Method: Protein A or G purified
Test Specificity: __