CD4 Monoclonal Antibody (YKIX302.9), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha
• This cell surface receptor is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha)
• In dogs, CD8 alpha is expressed on thymocytes, a subset of mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs)
• CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation
• This monoclonal antibody has been reported to inhibit cytotoxic T cell generation and function in vitro
• In addition, this antibody has been reported to be immunosuppressive in vivo
• Applications Reported: This YCATE55.9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This YCATE55.9 antibody has been pre-diluted and tested by flow cytometric analysis of normal canine peripheral blood cells
• This may be used at 5 μL (1 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
• We recommend using a 710/50 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
• CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
• At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
• CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
• It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].