7145108
CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Super Bright™ 436, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD49d (Integrin alpha 4)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias
269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; IA4; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin subunit alpha 4; integrin, alpha 4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); Itga4; Itga4B; ITGA-IV; LPAM subunit alpha; lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
Gene Symbols
ITGA4
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
ITGA4
Clone
R1-2
Applications
Flow Cytometry
Conjugate
Super Bright 436
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q00651
Gene ID (Entrez)
16401
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Description
- Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit
- The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106)
- This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils
- CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1)
- Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis
- Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
- We recommend using a 450/50 bandpass filter, or equivalent
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
- SB-4400) to minimize any non-specific polymer interactions
- The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins
- Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains
- Alpha4 chain associates with either beta1 or beta7 chain
- It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain
- These ligands included Madcam, VCAM, and fibronectin
- Madcam is known as the principal ligand for integrin alpha4beta7
- Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
Compare Similar Items
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Antigen: CD49d (Integrin alpha 4)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; IA4; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin subunit alpha 4; integrin, alpha 4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); Itga4; Itga4B; ITGA-IV; LPAM subunit alpha; lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
Gene Symbols: ITGA4
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: ITGA4
Clone: R1-2
Applications: Flow Cytometry
Conjugate: Super Bright 436
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q00651
Gene ID (Entrez): 16401
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD49d (Integrin alpha 4)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; IA4; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin subunit alpha 4; integrin, alpha 4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); Itga4; Itga4B; ITGA-IV; LPAM subunit alpha; lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
Gene Symbols:
ITGA4
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
ITGA4
Clone:
R1-2
Applications:
Flow Cytometry
Conjugate:
Super Bright 436
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q00651
Gene ID (Entrez):
16401
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD61 (Integrin beta 3)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: antigen CD61; BDPLT16; BDPLT2; beta3 integrin; CD61; EGK_08849; glycoprotein GPIIIa; glycoprotein IIIa; GP3A; GPIIIa; GT; INGRB3; integrin beta 3; integrin beta 3 (Cd61); integrin beta-3; integrin beta-3 subunit; integrin subunit beta 3; integrin, beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGB3; platelet glycoprotein IIIa; platelet glycoprotein IIIa integrin subunit beta 3; platelet gpIIIa; platelet membrane glycoprotein IIIa
Gene Symbols: ITGB3
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: ITGB3
Clone: VI-PL2
Applications: Flow Cytometry
Conjugate: APC-eFluor 780
Host Species: Mouse
Target Species: Baboon, Canine, Cynomolgus Monkey, Human, Rhesus Monkey
Gene Accession No.: P05106
Gene ID (Entrez): 102117791, 3690, 403788, 717379
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD61 (Integrin beta 3)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
antigen CD61; BDPLT16; BDPLT2; beta3 integrin; CD61; EGK_08849; glycoprotein GPIIIa; glycoprotein IIIa; GP3A; GPIIIa; GT; INGRB3; integrin beta 3; integrin beta 3 (Cd61); integrin beta-3; integrin beta-3 subunit; integrin subunit beta 3; integrin, beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGB3; platelet glycoprotein IIIa; platelet glycoprotein IIIa integrin subunit beta 3; platelet gpIIIa; platelet membrane glycoprotein IIIa
Gene Symbols:
ITGB3
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
ITGB3
Clone:
VI-PL2
Applications:
Flow Cytometry
Conjugate:
APC-eFluor 780
Host Species:
Mouse
Target Species:
Baboon, Canine, Cynomolgus Monkey, Human, Rhesus Monkey
Gene Accession No.:
P05106
Gene ID (Entrez):
102117791, 3690, 403788, 717379
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: IgE
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias: HCAK1; IgA1; IgG3; IGHA1; IGHA2; IGHG1; IGHG2; IGHG3; IGHG4; IGKC; IGKCD; IGLC; IGLC1; Immunoglobulin E; immunoglobulin heavy constant alpha 1; immunoglobulin heavy constant alpha 2 (A2m marker); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); immunoglobulin kappa constant; immunoglobulin lambda constant 1; ImmunoglobulinE; Km
Gene Symbols: IGHE
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: IGHE
Clone: Ige21
Applications: Flow Cytometry
Conjugate: Super Bright 600
Host Species: Mouse
Target Species: Human
Gene Accession No.: P01854
Gene ID (Entrez): 3497
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
IgE
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
HCAK1; IgA1; IgG3; IGHA1; IGHA2; IGHG1; IGHG2; IGHG3; IGHG4; IGKC; IGKCD; IGLC; IGLC1; Immunoglobulin E; immunoglobulin heavy constant alpha 1; immunoglobulin heavy constant alpha 2 (A2m marker); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); immunoglobulin kappa constant; immunoglobulin lambda constant 1; ImmunoglobulinE; Km
Gene Symbols:
IGHE
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
IGHE
Clone:
Ige21
Applications:
Flow Cytometry
Conjugate:
Super Bright 600
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P01854
Gene ID (Entrez):
3497
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: T-bet
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: HGNC:11599; LOW QUALITY PROTEIN: T-box transcription factor TBX21; Tbet; T-bet; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM; transcription factor T-box 21
Gene Symbols: Tbx21
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Tbx21
Clone: eBio4B10 (4B10)
Applications: Flow Cytometry
Conjugate: APC
Host Species: Mouse
Target Species: Human, Mouse, Rhesus Monkey
Gene Accession No.: Q9JKD8, Q9UL17
Gene ID (Entrez): 30009, 57765, 694044
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
T-bet
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
HGNC:11599; LOW QUALITY PROTEIN: T-box transcription factor TBX21; Tbet; T-bet; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM; transcription factor T-box 21
Gene Symbols:
Tbx21
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Tbx21
Clone:
eBio4B10 (4B10)
Applications:
Flow Cytometry
Conjugate:
APC
Host Species:
Mouse
Target Species:
Human, Mouse, Rhesus Monkey
Gene Accession No.:
Q9JKD8, Q9UL17
Gene ID (Entrez):
30009, 57765, 694044
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody