CD23 Monoclonal Antibody (EBVCS2), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein
• CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets
• Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active
• CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation
• CD21 and the alpha subunit of CD11b and CD11c bind to CD23
• Applications Reported: This EBVCS2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This EBVCS2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
• We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
•  Please make sure that your instrument is capable of detecting this fluorochrome
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
• The CD23 molecule is identical to the low affinity IgE receptor found on B cells
• Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma
• CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts
• Functionally, CD23 is involved in B cell growth and differentiation, and IgE production
• Further, CD23 has a soluble form that is a potent mitogenic factor
• Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.