CD3 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: SK7, BD Horizon™

Description

• The SK7 (Leu-4) monoclonal antibody specifically binds to the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex
• This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa
• The antigen recognized by CD3 antibodies is noncovalently associated with either α / β or γ/ δ TCR (70 to 90 kDa)
• The CD3 antigen is present on 61% to 85% of normal peripheral blood lymphocytes 60% to 85% of thymocytes and on Purkinje cells in the cerebellum
• The soluble form of this antibody has a mitogenic effect on most peripheral blood T lymphocytes, provided appropriate functional monocytes are present
• Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• Different BUV737 reagents (e.g., CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.