CD4 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: SK3 (also known as Leu3a), BD Horizon™

Description

• The SK3 monoclonal antibody specifically binds to CD4
• The CD4 antigen is a 55 kDa type 1 transmembrane glycoprotein that belongs to the immunoglobulin superfamily
• It is expressed on T-helper/inducer lymphocytes and monocytes
• CD3+CD4+ T cells comprise approximately 28% to 58% of normal peripheral blood lymphocytes
• CD4 is also expressed on 80% to 95% of normal thymocytes
• The CD4 antigen is present in low density on the cell surface of monocytes and in the cytoplasm of monocytes and macrophages (CD3-CD4+)
• CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV)
• The SK3 antibody inhibits HIV binding to CD4+ cells
• The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).