7162160
CD33 Monoclonal Antibody (9A11-CD33), Alexa Fluor™ 647, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD33
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide, pH 7.2
Gene Alias
CD33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, CD33 molecule transcript, FLJ00391, gp67, Myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, sialic acid-binding Ig-like lectin 3, SIGLEC3, Siglec-3
Gene Symbols
CD33
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
CD33
Clone
9A11-CD33
Applications
Flow Cytometry
Conjugate
Alexa Fluor 647
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q63994
Gene ID (Entrez)
12489
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Description
- Description: This monoclonal antibody 9A11-CD33 reacts with mouse CD33, also known as Siglec3 and gp67
- CD33 belongs to the sialic acid-binding immunoglobulin lectins (Siglec) family that binds to sialic acid-containing glycans on cell surface glycoproteins and glycolipids on mammalian cells
- Mouse CD33 binds to short O-linked glycans of certain mucins and sialyl-Tn epitopes
- Mouse CD33 is expressed by mature granulocytes, immature myeloid cells, and microglia
- Unlike human CD33, mouse CD33 lacks a cytoplasmic ITIM domain and exists as two splice variants that have different cytoplasmic tails
- In microglia, CD33 inhibits the phagocytosis of toxic amyloid beta
- Clone 9A11 has been used to characterize mouse CD33 surface expression and function
- <a href=https://www.ncbi.nlm.nih.gov/pubmed/31925286>View Article</a>
- Applications Reported: This 9A11-CD33 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 9A11-CD33 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This may be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family
- It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface
- CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia
- Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells
- CD33 is also expressed on, and is a useful marker for, peripheral monocytes
- CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias
- Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
Compare Similar Items
Show Difference
Antigen: CD33
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide, pH 7.2
Gene Alias: CD33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, CD33 molecule transcript, FLJ00391, gp67, Myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, sialic acid-binding Ig-like lectin 3, SIGLEC3, Siglec-3
Gene Symbols: CD33
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: CD33
Clone: 9A11-CD33
Applications: Flow Cytometry
Conjugate: Alexa Fluor 647
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q63994
Gene ID (Entrez): 12489
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD33
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide, pH 7.2
Gene Alias:
CD33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, CD33 molecule transcript, FLJ00391, gp67, Myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, sialic acid-binding Ig-like lectin 3, SIGLEC3, Siglec-3
Gene Symbols:
CD33
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
CD33
Clone:
9A11-CD33
Applications:
Flow Cytometry
Conjugate:
Alexa Fluor 647
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q63994
Gene ID (Entrez):
12489
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD366 (TIM3)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Alias: CD366, FLJ14428, Havcr2, HAVcr-2, Hepatitis A virus cellular receptor 2, hepatitis A virus cellular receptor 2 homolog, kidney injury molecule-3, KIM-3, sCD366, soluble CD366, soluble TIM 3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, Tim3, TIM-3, TIMD3, TIMD-3
Gene Symbols: Havcr2
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Havcr2
Clone: F38-2E2
Applications: Flow Cytometry
Conjugate: PE-Cyanine5
Host Species: Mouse
Target Species: Human
Gene Accession No.: Q8TDQ0
Gene ID (Entrez): 84868
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD366 (TIM3)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Alias:
CD366, FLJ14428, Havcr2, HAVcr-2, Hepatitis A virus cellular receptor 2, hepatitis A virus cellular receptor 2 homolog, kidney injury molecule-3, KIM-3, sCD366, soluble CD366, soluble TIM 3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, Tim3, TIM-3, TIMD3, TIMD-3
Gene Symbols:
Havcr2
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Havcr2
Clone:
F38-2E2
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine5
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
Q8TDQ0
Gene ID (Entrez):
84868
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD366 (TIM3)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide, pH 7.2
Gene Alias: CD366, FLJ14428, Havcr2, HAVcr-2, Hepatitis A virus cellular receptor 2, hepatitis A virus cellular receptor 2 homolog, kidney injury molecule-3, KIM-3, sCD366, soluble CD366, soluble TIM 3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, Tim3, TIM-3, TIMD3, TIMD-3
Gene Symbols: Havcr2
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Havcr2
Clone: RMT3-23
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q8VIM0
Gene ID (Entrez): 171285
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD366 (TIM3)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide, pH 7.2
Gene Alias:
CD366, FLJ14428, Havcr2, HAVcr-2, Hepatitis A virus cellular receptor 2, hepatitis A virus cellular receptor 2 homolog, kidney injury molecule-3, KIM-3, sCD366, soluble CD366, soluble TIM 3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, Tim3, TIM-3, TIMD3, TIMD-3
Gene Symbols:
Havcr2
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Havcr2
Clone:
RMT3-23
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q8VIM0
Gene ID (Entrez):
171285
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD4
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Alias: Activation B7-1 antigen, B7, B7.1, B7-1, BB1, B-lymphocyte activation antigen B7, CD28LG, CD28LG1, CD4, CD4 antigen, CD4 antigen (p55), CD4 antigen p55, Cd4 molecule, CD4 precursor, CD4 receptor, CD4, allele 1, cd4a, CD4mut, CD80, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CD80 molecule, cell surface glycoprotein CD4, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, fCD4, L3T4, LAB7, Leu-3, Ly-4, lymphocyte antigen CD4, lymphocyte antigen CD4 precursor, membrane protein, p55, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4), T-lymphocyte activation antigen CD80, W3/25, W3/25 antigen
Gene Symbols: CD4
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: CD4
Clone: RPA-T4
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Mouse
Target Species: Human
Gene Accession No.: P01730
Gene ID (Entrez): 920
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD4
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide, pH 7.2
Gene Alias:
Activation B7-1 antigen, B7, B7.1, B7-1, BB1, B-lymphocyte activation antigen B7, CD28LG, CD28LG1, CD4, CD4 antigen, CD4 antigen (p55), CD4 antigen p55, Cd4 molecule, CD4 precursor, CD4 receptor, CD4, allele 1, cd4a, CD4mut, CD80, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CD80 molecule, cell surface glycoprotein CD4, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, fCD4, L3T4, LAB7, Leu-3, Ly-4, lymphocyte antigen CD4, lymphocyte antigen CD4 precursor, membrane protein, p55, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4), T-lymphocyte activation antigen CD80, W3/25, W3/25 antigen
Gene Symbols:
CD4
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
CD4
Clone:
RPA-T4
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P01730
Gene ID (Entrez):
920
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody