7162926
Chlamydial LPS, Mouse anti-Bacteria, Clone: EVI-H1, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Chlamydial LPS
Dilution
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Bacteria
Gene Alias
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Isotype
IgG2a
Purification Method
Protein G purified
Test Specificity
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Clone
EVI-H1
Applications
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Chlamydial LPS, clone EVI-H1, Cat
- No
- MABF2107, is a mouse monoclonal antibody that detects Chlamydial lipopolysaccharide (LPS) and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunofluorescence, and Western Blotting
- Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV)
- Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants
- These are identified based on serological reactivity of the epitopes on their outer membrane
- Intracellularly chlamydia replicates within a vacuole
- Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles
- This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell
- Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate
- Lipopolysaccharides (LPS) of Chlamydia consists of at least three antigen domains, two of which are shared by the LPS of certain other gram-negative organisms and one that is unique only to chlamydial LPS
- It has been reported that the quantitative yields of chlamydial LPS can be achieved when elementary bodies (EBs) are first reduced and alkylated prior to extraction with hot phenol-water
- (Ref.: Caldwell, HD., and Hitchcock, PJ
- (1984)
- Infect
- Immun
- (44(2); 306-314; Scidmore, MA., et al
- (1996)
- Infect
- Immun
- 64(12); 5366-5372; Turingan, RS et al
- (2017)
- PLoS ONE 12(5): e0178653).
Compare Similar Items
Show Difference
Antigen: Chlamydial LPS
Dilution: Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Isotype: IgG2a
Purification Method: Protein G purified
Test Specificity: Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Clone: EVI-H1
Applications: Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen: Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial LPS
Dilution:
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Isotype:
IgG2a
Purification Method:
Protein G purified
Test Specificity:
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Clone:
EVI-H1
Applications:
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen:
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Chlamydial HSP60
Dilution: Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia heat shock protein 60
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Clone: A57-B9
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen: Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial HSP60
Dilution:
Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia heat shock protein 60
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Clone:
A57-B9
Applications:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen:
Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD318 (CD6L)
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone: 3A11
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen: HBL-100 human mammary gland epithelial cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CD318 (CD6L)
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone:
3A11
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen:
HBL-100 human mammary gland epithelial cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD318 (CD6L)
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone: 3A11
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen: HBL-100 human mammary gland epithelial cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CD318 (CD6L)
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone:
3A11
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen:
HBL-100 human mammary gland epithelial cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.