7162929
CD318 (CD6L), Mouse anti-Human, Clone: 3A11, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD318 (CD6L)
Dilution
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Gene Symbols
CDCP1;TRASK
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone
3A11
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_073753.3
Immunogen
HBL-100 human mammary gland epithelial cells.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-CD318 (CD6L), clone 3A11, Cat
- No
- MABF2119, is a mouse monoclonal antibody that detects CD318 (CD6L) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting
- CUB domain-containing protein 1 (UniProt: Q9H5V8; also known as Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with Src kinases, CD318, CD6L) is encoded by the CDCP1 (also known as TRASK) gene (Gene ID:64866) in human
- CD318 is a single-pass membrane protein (isoform 1) that serves as a ligand for CD6
- It is highly expressed in mitotic cells and has low expression during interphase
- It is detected in high levels in skeletal muscle and colon tissue and in lower levels in kidney, placenta, lungs, and small intestine
- Its levels are shown to be up-regulated in several human cancer tissues
- It is also expressed in high levels in synovial tissues where it participates in CD6-dependent adhesion of T cells to synovial fibroblasts
- CD318 is shown to be chemoattractive to T cells and levels of soluble CD318 are increased in the synovial fluid from patients with rheumatoid arthritis and juvenile inflammatory arthritis
- CD318 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form
- It has an extracellular domain (aa 30-667), a transmembrane domain (aa 668-688), and a cytoplasmic domain (aa 689-836)
- Three isoforms of CD318 have been described that are produced by alternative splicing
- (Ref.: Enyindah-Asonye, G., et al
- (2017)
- Proc
- Natl
- Acad
- Sci
- USA 114(33); E6912-E6921).
Compare Similar Items
Show Difference
Antigen: CD318 (CD6L)
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Gene Symbols: CDCP1;TRASK
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone: 3A11
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_073753.3
Immunogen: HBL-100 human mammary gland epithelial cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CD318 (CD6L)
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD6L
Gene Symbols:
CDCP1;TRASK
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
Clone:
3A11
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_073753.3
Immunogen:
HBL-100 human mammary gland epithelial cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: PCBP2 (hnRNP E2)
Dilution: Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).Western Blotting Analysis: 1μg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Poly(rC)-binding protein 2;Alpha-CP2;Heterogeneous nuclear ribonucleoprotein E2;hnRNP E2
Gene Symbols: PCBP2
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
Clone: PC 81-1
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001122383
Immunogen: His-tagged full-length human recombinant Poly(rC)-binding protein 2.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
PCBP2 (hnRNP E2)
Dilution:
Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).Western Blotting Analysis: 1μg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Poly(rC)-binding protein 2;Alpha-CP2;Heterogeneous nuclear ribonucleoprotein E2;hnRNP E2
Gene Symbols:
PCBP2
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
Clone:
PC 81-1
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001122383
Immunogen:
His-tagged full-length human recombinant Poly(rC)-binding protein 2.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: chlamydial MOMP
Dilution: Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81). Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia major outer membrane protein
Gene Symbols: N/A
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.
Clone: B-D3
Applications: Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez): __
Immunogen: Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
chlamydial MOMP
Dilution:
Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81). Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia major outer membrane protein
Gene Symbols:
N/A
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.
Clone:
B-D3
Applications:
Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez):
__
Immunogen:
Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Aminopeptidase N (CD13)
Dilution: Immunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. al. (2014). Front Physiol. 4:402; Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. al. (2014). Front Physiol. 4:402).Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: AP-N;hAPN;Alanyl aminopeptidase;Aminopeptidase M;AP-M;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13;gp150;CD13
Gene Symbols: ANPEP;APN;CD13;PEPN
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 452 specifically detects Aminopeptidase N (CD13) in human cells.
Clone: 452
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez): NP_001141
Immunogen: Isolated human dermal fibroblasts.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
Aminopeptidase N (CD13)
Dilution:
Immunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. al. (2014). Front Physiol. 4:402; Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. al. (2014). Front Physiol. 4:402).Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
AP-N;hAPN;Alanyl aminopeptidase;Aminopeptidase M;AP-M;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13;gp150;CD13
Gene Symbols:
ANPEP;APN;CD13;PEPN
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 452 specifically detects Aminopeptidase N (CD13) in human cells.
Clone:
452
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez):
NP_001141
Immunogen:
Isolated human dermal fibroblasts.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.