7162931
chlamydial MOMP, Mouse anti-Bacteria, Clone: B-D3, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
chlamydial MOMP
Dilution
Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81). Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Bacteria
Gene Alias
Chlamydia major outer membrane protein
Immunogen
Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone
B-D3
Applications
Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene Symbols
N/A
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.
Description
- Anti-chlamydial MOMP, clone B-D3, Cat
- No
- MABF2129, is a mouse monoclonal antibody that detects Chlamydial major outer membrane protein (MOMP) and has been tested for use in Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Neutralizing applications
- Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV)
- Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants
- These are identified based on serological reactivity of the epitopes on their outer membrane
- Intracellularly chlamydia replicates within a vacuole
- Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles
- This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell
- Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate
- Clone B-D3 specifically detects surface exposed epitopes on major outer membrane protein (MOMP) from Chlamydia genus
- It displays strong reactivity with Chlamydial serovars B, Ba, D, E, and L2 and weak reactivity with L1
- MOMP, a cysteine-rich molecule, plays a vital role in maintaining the structural and functional properties of the chlamydial outer membrane
- It contains determinants that confer type, subspecies, and species antigenic properties to the protein
- It displays high sensitivity to heat and trypsin treatment that destroys MOMP epitopes recognized by this clone
- (Ref.: Zhang, YX et al., (1987)
- J
- Immunol
- 138(2); 575-581).
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Antigen: chlamydial MOMP
Dilution: Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81). Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia major outer membrane protein
Immunogen: Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone: B-D3
Applications: Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene Symbols: N/A
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.
Antigen:
chlamydial MOMP
Dilution:
Neutralizing Analysis: A representative lot neutralized Chlamydia and prevented toxicity in murine model (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81). Immunoprecipitation Analysis: A representative lot immunoprecipitated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected Chlamydial MOMP in McCoy cells infected with LGV serovar L2 inclusions (Courtesy of Chunfu Yang, Ph.D. and Harlan D. Caldwell, Ph.D., National Institutes of Health, Bethesda, MD USA).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia major outer membrane protein
Immunogen:
Formalin killed Chlamydia trachornatis serovar L2 (LGV 434).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone:
B-D3
Applications:
Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene Symbols:
N/A
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone B-3D detects Chlamydial major outer membrane protein. It displays reactivity to serovars B, Ba, D, E, and L2 and weak reactivity with L1.
Antigen: Aminopeptidase N (CD13)
Dilution: Immunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. al. (2014). Front Physiol. 4:402; Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. al. (2014). Front Physiol. 4:402).Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: AP-N;hAPN;Alanyl aminopeptidase;Aminopeptidase M;AP-M;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13;gp150;CD13
Immunogen: Isolated human dermal fibroblasts.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone: 452
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene Symbols: ANPEP;APN;CD13;PEPN
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 452 specifically detects Aminopeptidase N (CD13) in human cells.
Antigen:
Aminopeptidase N (CD13)
Dilution:
Immunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. al. (2014). Front Physiol. 4:402; Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. al. (2014). Front Physiol. 4:402).Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
AP-N;hAPN;Alanyl aminopeptidase;Aminopeptidase M;AP-M;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13;gp150;CD13
Immunogen:
Isolated human dermal fibroblasts.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone:
452
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene Symbols:
ANPEP;APN;CD13;PEPN
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 452 specifically detects Aminopeptidase N (CD13) in human cells.
Antigen: TMPRSS2
Dilution: Immunohistochemistry (Paraffin) Analysis: 1:50 dilution from a representative lot detected TMPRSS2 in human kidney tissue sections.Enzyme Immunoassay (ELISA) Analysis: A representative lot detected TMPRSS2 in ELISA applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Western Blotting Analysis: A representative lot detected TMPRSS2 in Western Blotting applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Immunohistochemistry (Paraffin) Analysis: A representative lot detected TMPRSS2 in Immunohistochemistry applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25; Bertram, S., et. al. (2012). PLoS One. 7(4):e35876).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Transmembrane protease serine 2;Serine protease 10
Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human transmembrane protease serine 2.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: P5H9-A3
Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: TMPRSS2;PRSS10
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone P5H9-A3 specifically detects human Transmembrane protease serine 2. It targets an epitope with in the extracellular serine protease domain.
Antigen:
TMPRSS2
Dilution:
Immunohistochemistry (Paraffin) Analysis: 1:50 dilution from a representative lot detected TMPRSS2 in human kidney tissue sections.Enzyme Immunoassay (ELISA) Analysis: A representative lot detected TMPRSS2 in ELISA applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Western Blotting Analysis: A representative lot detected TMPRSS2 in Western Blotting applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25).Immunohistochemistry (Paraffin) Analysis: A representative lot detected TMPRSS2 in Immunohistochemistry applications (Lucas, J.M., et. al. (2008). J Pathol. 215(2):118-25; Bertram, S., et. al. (2012). PLoS One. 7(4):e35876).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Transmembrane protease serine 2;Serine protease 10
Immunogen:
KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human transmembrane protease serine 2.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
P5H9-A3
Applications:
ELISA, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
TMPRSS2;PRSS10
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone P5H9-A3 specifically detects human Transmembrane protease serine 2. It targets an epitope with in the extracellular serine protease domain.
Antigen: CD63 (LAMP3)
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections.Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81).Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70).Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD63 antigen;Granulophysin;Lysosomal-associated membrane protein 3;LAMP-3;Melanoma-associated antigen ME491;OMA81H;Ocular melanoma-associated antigen;Tetraspanin-30;Tspan-30
Immunogen: Clear supernatant from SK-Mel-23 cell lysate.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: ME491
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: CD63;MLA1;TSPAN30
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone ME491 specifically detects CD63 (LAMP-3) in human cells.
Antigen:
CD63 (LAMP3)
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections.Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81).Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70).Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD63 antigen;Granulophysin;Lysosomal-associated membrane protein 3;LAMP-3;Melanoma-associated antigen ME491;OMA81H;Ocular melanoma-associated antigen;Tetraspanin-30;Tspan-30
Immunogen:
Clear supernatant from SK-Mel-23 cell lysate.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
ME491
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
CD63;MLA1;TSPAN30
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone ME491 specifically detects CD63 (LAMP-3) in human cells.