7162934
CD63 (LAMP3), Mouse anti-Human, Clone: ME491, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD63 (LAMP3)
Dilution
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections.Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81).Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70).Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
CD63 antigen;Granulophysin;Lysosomal-associated membrane protein 3;LAMP-3;Melanoma-associated antigen ME491;OMA81H;Ocular melanoma-associated antigen;Tetraspanin-30;Tspan-30
Gene Symbols
CD63;MLA1;TSPAN30
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone ME491 specifically detects CD63 (LAMP-3) in human cells.
Clone
ME491
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001244318
Immunogen
Clear supernatant from SK-Mel-23 cell lysate.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Related Products
Description
- Anti-CD63 (LAMP3), clone ME491, Cat
- No
- MABF2159, is a mouse monoclonal antibody that detects CD63 antigen and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting
- CD63 antigen (UniProt: P08962; also known as Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63) is encoded by the CD63 (also known as MLA1, TSPAN30) gene (Gene ID: 967) in human
- CD63 is a multi-pass membrane protein of the tetraspan family that is found on endosome, lysosome, and plasma membranes
- CD63 has been detected in platelets, Dysplastic nevi benign moles), radial growth phase primary melanomas, hematopoietic cells, and in tissue macrophages
- In melanoma cells it is involved in their motility and adhesion
- CD63 also plays a role in the adhesion of leukocytes onto endothelial cells
- It is reported to play a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases and promote cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration
- CD63 is a highly N-glycosylated protein with three asparagine glycosylation sites (aa 130, 150, 172) and its ribophorin II (RPN2)-mediated glycosylation has been linked to breast cancer
- Overexpression of CD63 has been observed in esophageal cancer that is negatively correlated with tumor stage and lymph node metastasis
- Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS), an autosomal recessive disorder that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects
- This antibody (clone ME491) is shown to react with human primary and to some extent with metastatic melanoma tissues
- (Ref.: Lai, X., et al
- (2017)
- Oncol
- Let
- 13(6); 4245-4251; Tominaga, N., et al
- (2014)
- Mol
- Cancer 13; 134; Smith, M., et al
- (1997)
- Melanoma Res
- 7 (Suppl
- 2), 163-170).
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Antigen: CD63 (LAMP3)
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections.Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81).Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70).Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CD63 antigen;Granulophysin;Lysosomal-associated membrane protein 3;LAMP-3;Melanoma-associated antigen ME491;OMA81H;Ocular melanoma-associated antigen;Tetraspanin-30;Tspan-30
Gene Symbols: CD63;MLA1;TSPAN30
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone ME491 specifically detects CD63 (LAMP-3) in human cells.
Clone: ME491
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001244318
Immunogen: Clear supernatant from SK-Mel-23 cell lysate.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
CD63 (LAMP3)
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections.Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81).Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70).Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
CD63 antigen;Granulophysin;Lysosomal-associated membrane protein 3;LAMP-3;Melanoma-associated antigen ME491;OMA81H;Ocular melanoma-associated antigen;Tetraspanin-30;Tspan-30
Gene Symbols:
CD63;MLA1;TSPAN30
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone ME491 specifically detects CD63 (LAMP-3) in human cells.
Clone:
ME491
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001244318
Immunogen:
Clear supernatant from SK-Mel-23 cell lysate.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: HLA-F
Dilution: ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Gene Symbols: HLA-F;HLA-5.4;HLAF
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Clone: 3D11
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_061823
Immunogen: Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
HLA-F
Dilution:
ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Gene Symbols:
HLA-F;HLA-5.4;HLAF
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Clone:
3D11
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_061823
Immunogen:
Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: CD33
Dilution: Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Gene Symbols: CD33;SIGLEC3
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone P67.6 specifically detects human CD 33.
Clone: P67.6
Applications: Flow Cytometry, Immunocytochemistry, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez): NP_001763.3
Immunogen: FMY9S5 cells expressing CD33.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD33
Dilution:
Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Gene Symbols:
CD33;SIGLEC3
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone P67.6 specifically detects human CD 33.
Clone:
P67.6
Applications:
Flow Cytometry, Immunocytochemistry, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez):
NP_001763.3
Immunogen:
FMY9S5 cells expressing CD33.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: Chlamydial MOMP
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydia major membrane protein
Gene Symbols: N/A
Isotype: IgG2a λ
Purification Method: Protein G purified
Test Specificity: Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
Clone: L21-10
Applications: Dot Blot, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Formalin killed Chlamydia trachomatis (L2/LGV-434).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial MOMP
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipiated Chlamydial MOMP in Immunoprecipitation applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Immunofluorescence Analysis: A representative lot detected Chlamydial MOMP in Immunofluorescence applications (Weber, M.M., et. al. (2017). Cell Rep. 19(7):1406-1417; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Western Blotting Analysis: A representative lot detected Chlamydial MOMP in Western Blotting applications (Baehr, W., et. al. (1988). Proc Natl Acad Sci USA. 85(11):4000-4; Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).Dot Blot Analysis: A representative lot detected Chlamydial MOMP in Dot Blot applications (Zhang, Y.X., et. al. (1987). J Immunol. 138(2):575-81).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydia major membrane protein
Gene Symbols:
N/A
Isotype:
IgG2a λ
Purification Method:
Protein G purified
Test Specificity:
Clone L21-10 specifically detects outer membrane protein from Chlamydia genus. It displays strong reactivity with serovars D and L3 and weak reactivity with Ba, E, G, L1, and L2.
Clone:
L21-10
Applications:
Dot Blot, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Formalin killed Chlamydia trachomatis (L2/LGV-434).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.