7162922
CD11b/CD18, Mouse anti-Human, Non-human Primate, Clone: mAb107, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD11b/CD18
Dilution
Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Non-human Primate
Gene Alias
Integrin alpha-M/ITGB2;CR3
Gene Symbols
ITGAM;CD11B;CR3A
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.
Clone
mAb107
Applications
Dot Blot, Flow Cytometry, Immunohistochemistry, Inhibition Assays
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Structure
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_000623
Immunogen
GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Related Products
Description
- Anti-CD11b/CD18, clone mAb107, Cat
- No
- MABF2085, is a mouse monoclonal antibody that detects CD11b/CD18 nd has been tested for use in Dot Blot, Flow Cytometry, Immunohistochemistry, and Inhibition assay
- Integrin alpha-M (UniProt: P11215; also known as CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b) is encoded by the ITGAM (also known as CD11B, CR3A) gene (Gene ID: 3684) in human
- Integrins are alpha/beta heterodimeric adhesion receptors that couple the extracellular matrix on other cells with the contractile cytoskeleton to transduce mechanochemical signals across the plasma membrane
- CD11b is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-16), which is cleaved off in the mature form
- It is predominantly expressed in monocytes and granulocytes and some expression is also observed in neutrophils
- It is usually found as a heterodimer of alpha and beta subunits and associates with Integrin beta-2 (ITGB2)
- Two isoforms of CD11b have been reported that are produced by alternative splicing
- CD11b contains a large extracellular domain (aa 17-1104), a short transmembrane domain (aa 1105-1128), and a cytoplasmic domain (aa 1129-1152)
- It has been implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles
- It contains a metal ion-dependent adhesion site (MIDAS) in the integrin A domain, which is essential for stabilization of domain A in high affinity state
- The major ligand recognition site of CD11b/CD18 has been mapped to the A-domain in the CD11b subunit (CD11bA)
- Both magnesium and calcium ions exhibit equal affinities for the inactive CD11bA, however, upon activation there is a 10-fold increase in the binding affinity of magnesium ions without affecting affinity of calcium ions
- Mutations in ITGAM gene are known to cause Systemic lupus erythematosus 6, which is characterized by wide range of system dysfunctions, principally involving skin, joints, kidneys and serosal membranes
- (Ref.: Zerria, K., et al
- (2006)
- Immunology 119(4); 431-440; Mahalingam, B., et al., (2011)
- J
- Immunol
- 187 (12); 6393-6401; Li, R., et al
- (2002)
- J
- Immunol
- 168 (3); 1219-1225.).
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Show Difference
Antigen: CD11b/CD18
Dilution: Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Non-human Primate
Gene Alias: Integrin alpha-M/ITGB2;CR3
Gene Symbols: ITGAM;CD11B;CR3A
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.
Clone: mAb107
Applications: Dot Blot, Flow Cytometry, Immunohistochemistry, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_000623
Immunogen: GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
CD11b/CD18
Dilution:
Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Non-human Primate
Gene Alias:
Integrin alpha-M/ITGB2;CR3
Gene Symbols:
ITGAM;CD11B;CR3A
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.
Clone:
mAb107
Applications:
Dot Blot, Flow Cytometry, Immunohistochemistry, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_000623
Immunogen:
GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: SLAMF7 (CD319)
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
Gene Symbols: SLAMF7;CS1;UNQ576/PRO1138
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
Clone: 162
Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez): NP_067004.3
Immunogen: Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
SLAMF7 (CD319)
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
Gene Symbols:
SLAMF7;CS1;UNQ576/PRO1138
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
Clone:
162
Applications:
Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez):
NP_067004.3
Immunogen:
Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: FcRH5
Dilution: Immunocytochemistry Analysis: A representative lot detected FcRH5 in Immunocytochemistry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols: FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone F56 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone: F56
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_112571.2
Immunogen: Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
FcRH5
Dilution:
Immunocytochemistry Analysis: A representative lot detected FcRH5 in Immunocytochemistry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols:
FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone F56 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone:
F56
Applications:
ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_112571.2
Immunogen:
Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: FcRH5
Dilution: Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols: FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone: F25
Applications: ELISA, Flow Cytometry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_112571.2
Immunogen: Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
FcRH5
Dilution:
Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols:
FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone:
F25
Applications:
ELISA, Flow Cytometry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_112571.2
Immunogen:
Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.