7162923
SLAMF7 (CD319), Mouse anti-Human, Clone: 162, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
SLAMF7 (CD319)
Dilution
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
Gene Symbols
SLAMF7;CS1;UNQ576/PRO1138
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
Clone
162
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Inflammation & Immunology
Formulation
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez)
NP_067004.3
Immunogen
Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Description
- Anti-SLAMF7 (CD319), clone 162, Cat
- No
- MABF2093, is a mouse monoclonal antibody that detects SLAM family member 7 and has been tested for use in Flow Cytometry, Function Analysis, Immunohistochemistry (Paraffin), Immunoprecipitation, and Inhibition studies
- SLAM family member 7 (UniProt: Q9NQ25; also known as SLAMF7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, CRACC, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CD319) is encoded by the SLAMF7 (also known as CS1, UNQ576/PRO1138) gene (Gene ID: 57823) in human
- SLAMF-7 is a single-pass type I membrane protein that is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea
- Expression has also been detected in multiple myeloma cells, NK cells, activated B-cells, and NK-cell line, but not in promyelocytic, B-, or T-cell lines
- It serves as a receptor on immune cells, including natural killer (NK) cells
- SLAMF7 is reported to mediate both activating and inhibitory effects on NK cells, depending on the expression of adaptor EAT-2
- In cells lacking EAT-2 it mediates inhibition via SHIP-1, which is recruited via Tyr 261 of SLAMF7
- SLAMF7 is shown to be involved in phagocytosis of hematopoietic tumor cells independent of signaling lymphocyte activation molecule-associated protein (SAP) adaptors, but depends on its ability to interact with integrin Mac-1 and utilizes signals involving ITSM motif (aa 302-307)
- SLAMF7 is synthesized with a signal peptide (aa 1-22), which is subsequently removed to generate the mature form that has an extracellular domain (aa 23-226), a short transmembrane domain (aa 227-247), and a cytoplasmic tail (aa 248-335)
- Seven different isoforms of SLAMF7 have been described that are produced by alternative splicing
- Clone 162 is shown to block the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to Anti-CD47 antibodies
- (Ref.: Guo, H., et al
- (2015)
- Mol
- Cell
- Biol
- 35 (1); 41-51; Chen, J., et al
- (2017)
- Nature 544 (7651); 493-497).
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Antigen: SLAMF7 (CD319)
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
Gene Symbols: SLAMF7;CS1;UNQ576/PRO1138
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
Clone: 162
Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez): NP_067004.3
Immunogen: Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
SLAMF7 (CD319)
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
Gene Symbols:
SLAMF7;CS1;UNQ576/PRO1138
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
Clone:
162
Applications:
Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Gene ID (Entrez):
NP_067004.3
Immunogen:
Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: FcRH5
Dilution: Immunocytochemistry Analysis: A representative lot detected FcRH5 in Immunocytochemistry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols: FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone F56 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone: F56
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_112571.2
Immunogen: Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
FcRH5
Dilution:
Immunocytochemistry Analysis: A representative lot detected FcRH5 in Immunocytochemistry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols:
FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone F56 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone:
F56
Applications:
ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_112571.2
Immunogen:
Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: FcRH5
Dilution: Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols: FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone: F25
Applications: ELISA, Flow Cytometry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_112571.2
Immunogen: Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
FcRH5
Dilution:
Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Fc receptor-like protein 5;FcR-like protein 5;FcRL5;BXMAS1;Fc receptor homolog 5;Immune receptor translocation-associated protein 2;IRTA2;CD307e
Gene Symbols:
FCRL5;FCRH5;IRTA2;UNQ503/PRO820
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.
Clone:
F25
Applications:
ELISA, Flow Cytometry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_112571.2
Immunogen:
Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Chlamydial LPS
Dilution: Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Gene Symbols: __
Isotype: IgG2a
Purification Method: Protein G purified
Test Specificity: Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Clone: EVI-H1
Applications: Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Chlamydial LPS
Dilution:
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Gene Symbols:
__
Isotype:
IgG2a
Purification Method:
Protein G purified
Test Specificity:
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Clone:
EVI-H1
Applications:
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.